However, less than 20 % of cats with heartworms have
microfilariae in their blood, and microfilariae are only present for one to four weeks.
A single mature female can produce up to 5,000
microfilariae in one day, each of which can survive in the dog's bloodstream for years, continuing to produce more microfilariae.
If the antigen screening test is positive, another blood test is available to identify the concentration of
microfilariae in circulation.
If this test is negative, the animal still might be infected, because up to 25 % of dogs with heartworm disease don't have identifiable
microfilariae in their blood.
Heartworm disease begins with an infected animal that has circulating
microfilariae in its blood.
As the mosquito stops by for a meal it also sucks up a number of circulating
microfilariae in the blood.
Nineteen cats from Tuscany, Umbria and Marche regions of Italy affected by pruritic dermatitis (Fig. 1) were found to carry Dirofilaria repens
microfilariae in the blood (Fig. 2), confirming that domestic felines are a valuable reservoir for infestation to humans in Central Italy.
Nonetheless, the detection of
microfilariae in the blood seems to be of significant diagnostic importance for the presence of adults in Dirofilaria infestations (Anon, 1998).
Clinical re-examination and a Knott test, carried out 1 month after the completion of treatment, showed the resolution of the cutaneous lesions and the absence of Dirofilaria repens
microfilariae in the blood.
Tarello W. (2002a) Dermatitis associated with Dirofilaria (Nochtiella) repens
microfilariae in dogs from Central Italy.
The antigen test will be positive even when there are
no microfilariae in the blood.
Detection of D. immitis
microfilariae in a seronegative dog, can occur when only a single gravid female lives in the pulmonary arteries of the host.
In a recent study from another non-endemic area (Munchen, Germany), among 72 imported or travelling dogs with D. immitis
microfilariae in the blood, and examined between 1993 - 96, 27 (37.5 %) resulted sero - negative with the ELISA test (Zahler et al., 1997), apparently confirming the results here reported.
Two are the others canine filariases releasing
microfilariae in the blood and described in Italy: Dirofilaria (Nochtiella) repens and Acanthocheilonema (syn.: Dipetalonema) reconditum (Pampiglione et al., 1995; Tarello, 1999).
In such cases, accurate concentrations tests, however, can demonstrate circulating
microfilariae in many dogs, avoiding wrong diagnosis and diffusion of dirofilariasis to newly colonized areas.
The problem with using the slow - kill technique to kill adult heartworms, experts say, is this: If there are
microfilariae in the infected dog that are resistant to the macrocyclic lactone drug used, those parasites will be transmitted to mosquitoes, potentially finding their way to a new dog in which to reproduce, increasing the risk to all dogs in the area.
McCall JW, Arther R, Davis W, Settje T. Safety and efficacy of 10 % imidacloprid + 2.5 % moxidectin for the treatment of Dirofilaria immitis circulating
microfilariae in experimentally infected dogs.
This test should be used on any dogs that are on Heartgard since they will not have
microfilariae in their bloodstream.
Before giving your dog any kind of heartworm drugs, you can do DNA testing to see if there are
any microfilariae in his system.
That means they work by paralyzing the nervous system of
the microfilariae in your dog's body.
Many veterinarians prefer to do both tests as the absence of
microfilariae in the blood does not necessarily mean that there are no adult worms in the heart.
Dogs can have
some microfilariae in their blood and worms in their lungs without manifesting the disease.
The filtration test finds
microfilariae in the blood; the occult test locates adult worms in the heart.
Development of
the microfilariae in the mosquito requires a temperature at or above 80 degrees Fahrenheit for about two weeks.
For this reason, it is virtually impossible to detect
microfilariae in an infected cat.
NOTE: All commercially available heartworm preventives act by wiping out
the microfilariae in the middle stages.
It will be positive even if the dog does not have
any microfilaria in the blood; this occurs about 20 % of the time.
However, starting prevention without testing can be harmful, because once the heartworms reach a certain point in development, the killing off of
the microfilaria in combination with juvenile or adult heartworm in the system can be damaging to your dog's cardiovascular system.
If your dog is getting heartworm prevention every month, it will kill
the microfilaria in his bloodstream before it can develop into adult reproductive worms.
In Germany, canine dirofilariasis has been discovered on routine blood tests for
microfilaria in 13 % of dogs with history of travel to Italy, Portugal, Spain and Corsica and in 10 % of dogs imported from Italy, Spain and Portugal (Schrey, 1996).
It is generally thought that concentration tests (Knott, filtration) are the most sensitive, because detection of
microfilaria in the blood is diagnostic for the presence of adults in the pulmonary arteries (BSAVA, 1998).
Earlier this year, I examined 29 young dogs presented to shelters for routine spay / neuter in Farmington and Aztec, New Mexico and found episcleral granulomas with nematodes in 3 dogs and
microfilaria in a skin snip biopsy from one of the dogs with episcleral nematodes.
Advantage Multi actually carries approval for use in heartworm positive dogs, meaning that it can be used to kill
microfilaria in an active heartworm infection.
Most veterinary clinics can run an Antigen test in the hospital to detect
microfilaria in dogs; however, detection of heartworm is more difficult in cats and requires an Antibody test that is usually sent to an outside laboratory.
Not exact matches
In the skin the larvae mate and form so - called new
microfilaria, which then reach the dog's bloodstream.
This point - of - care device automatically captures and analyzes videos of microfilarial motion
in whole blood using motorized sample scanning and onboard motion detection, minimizing input from health care workers and providing a quantification of
microfilariae per milliliter of whole blood
in under 2 min.
Sentinel and Interceptor provide a similar warning to Heargard about mild hypersensitivity reactions
in a pet carrying high levels of
microfilariae.
However, the
microfilariae are seen
in greater numbers
in the summer months and
in the evening, so these variations must be considered.
These
microfilaria live
in the bloodstream, mainly
in the small blood vessels.
Products containing selamectin and moxidectin (Revolution and Proheart 6) are not effective
in clearing
microfilariae but will kill L3 and L4 larvae.
An occult heartworm infection means there's an infection of some sort but the
microfilariae, or the heartworm offspring, aren't found circulating around
in the blood.
It takes about 6 months for
microfilariae to develop into adult heartworms and once they mature, heartworms can only survive
in their host for 3 to 5 years.
«Single sex heartworm infections, host immune responses affecting the presence of circulating
microfilariae and the administration of heartworm preventives can be factors which produce occult infections
in dogs.»
If the
microfilariae are lucky enough to be hanging around an area
in the dog that's bitten by a second mosquito, and that mosquito lives long enough with a high temperature, the
microfilariae can develop into L3 larvae.
The
microfilariae develop further for 10 to 30 days
in the mosquito and then enter the mouth parts of the mosquito.
In this final stage, the
microfilariae migrates to the heart and out into the pulmonary arteries (if there is room) where it will mate, approximately 5 - 7 months after first entering the new host.
Once
in the bloodstream of the new host, the
microfilariae will spend the next week or two developing into the next stage of development within the host's skin.
Treating canine heartworm disease involves killing the adult worms that live
in the heart and pulmonary arteries, as well as those
in the larval stages (called
microfilaria) that circulate
in the bloodstream of the dog.
If your pet is off Sentinel for 6 months or less, re-start the medication and test after 6 months since
microfilaria, that take 6 months to mature, will result
in a positive test result.
In heartworms, a mosquito serves as the intermediate host for the larval stage of the worm, also known as the
microfilariae.