Sentences with word «microinjection»

17 Capecchi MR. High Efficiency Transformation By Direct Microinjection of DNA into Cultured Mammalian Cells.
We offer quality custom generation of a wide range of mouse models developed by microinjection of Sv129 / Pas or C57BL / 6N ES cells into blastocysts or by DNA pronuclear injection.
This will involve labeling cells in the growing embryo tail and using microinjections of dye to follow the pathways that these cells take as the tail develops.
High efficiency transformation by direct microinjection of DNA into cultured mammalian cells.
The CRB Xénopes (Xenopus Biological Resource Center) wants to develop a semiautomatic microinjection platform with two major objectives:
Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided.
Generation of genetically modified mice by random insertion of a transgene can be performed by pronuclear DNA microinjection.
JAX Transgenic Model Generation service includes pronuclear microinjection of your transgenic DNA (plasmid or BAC) into pronuclear embryos, who are then transferred into pseudopregnant females.
Not only was I miserable performing microinjections and single - cell transplants in a dark, isolated room but I also realized within 2 months that I was, without a doubt, ready to move on to something else.
Compared to conventional microinjection, the electroporation method was easy to carry out, fast and scalable.
The researchers also surveyed hypothalamic gene expression in mice derived from the zygote microinjections «The hypothalamus is what determines everything about how you survive: your eating, your sleeping, your circadian rhythms, your stress, your reproduction,» Bale said.
The fruitfly Drosophilia melanogaster is a model organism well suited for transgenesis and direct dsRNA microinjection.
«Conventional techniques of producing transgenic animals, such as microinjection of genes into eggs and the retroviral transduction of genes into embryos, often produce many animals that are mosaic, which means they do not contain the foreign gene in all their cells.
Generating mutations via direct Cas9 mRNA injection into embryos requires the equipment and expertise for embryo microinjection, which may not be readily available to all researchers.
These 2D cultures also abolish the need for intraluminal microinjection of single organoids and facilitate studies of host - microbe or virus interactions or the action of microbial enterotoxins because the luminal surface is fully exposed to the mucosal medium (22,23).
JAX has generated hundreds of transgenic mouse models in diverse backgrounds using traditional DNA pronuclear microinjection technology.
A flat tip microinjection pipette with an internal diameter of 15 — 18 µm was used for iPS cell injection.
Using microinjection of adeno - associated viral vector bearing a transgene encoding FKBP1b into the hippocampus of aged male rats, we assessed the critical prediction that overexpressing FKBP1b should reverse Ca2 + - mediated manifestations of brain aging.
This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.
The resulting gradients must drive a sizable centripetal flux of cAMP because direct microinjection of cAMP showed that it diffused readily.
Afterwards, anterograde connections from the retina were mapped by microinjection of 5 µl Choleratoxin B subunit (CtB, working dilution: 1 % in distilled water; Sigma, Deissenhofen, Germany) in the vitreous of the eye contralateral to the Wulst injection.
Microinjection, however, is an inefficient process.
The new cell extraction method is based on a microinjection system developed at ETH over the past years, the FluidFM, which is the «world's smallest automated syringe.»
This «microinjection» approach was pioneered by a team in Hawaii to produce Cumulina, the first cloned mouse (ScienceNOW, 22 July 1998).
The processes of microinjection and micromanipulation of egg and sperm are making a wider array of new treatments possible.
«It proves that microinjection can be used in large animals and livestock.»
The level of fluorescence you saw in the validation assay should give an accurate indication of how many pups will contain a mutation and many of the mice with the mutation will be homozygous for it in every cell; however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice.
The new device builds on previous work by Jensen and Langer's labs, in which they used microinjection to force large molecules into cells as they flowed through a microfluidic device.
JAX will deliver all the animals with confirmed genetic modifications resulting from the microinjection.
The Department houses the microinjection platform which provides services in DNA and ES cell injections, and mouse cryopreservation.
Microinjection of mRNA enhances translational efficiency of human adenovirus fiber message in monkey cells
Microinjection of membrane - impermeable molecules into single neural stem cells in brain tissue.
This technology requires technical skills that are typically not offered by veterinary practices and includes aspiration of immature or mature eggs from mares using ultrasound - guided transvaginal aspiration (TVA) of follicles, in vitro culture of the eggs, micromanipulation and microinjection of eggs with a single selected sperm, and embryo culture in the laboratory, with freezing, and transfer of embryos to synchronized recipient mares.
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