The reactions converted nonfluorescent reactants to highly fluorescent products, allowing the researchers to watch the process unfold using high - resolution fluorescence
microscopy methods.
Brunger also employs single - molecule fluorescence
microscopy methods to complement his structural studies.
Other areas of instrument development include DNA sequencing, cell fractionation, light and electron
microscopy methods, mass spectrometry of proteins, X-ray imaging plates, synchrotron beam - lines and automated cell micro injectors.
«This technique also later aided the development of several super-resolution
microscopy methods, which earned other researchers a Nobel Prize in chemistry in 2014,» says Yildiz.
Article highlights
microscopy methods that show the formation of bone tissue, battery shards, and more
Development of methodology to facilitate single cell studies of structural variation, and integrating state - of - the - art
microscopy methods with single cell sequencing.
Results: Researchers at Pacific Northwest National Laboratory have for the first time integrated
microscopy methods with controlled cultivation to monitor microbial physiology in live bacterial biofilms.
The main significance of the team's new tool is that «the RAMS load frame insert enables applying axial loads while the specimen is continuously rotated, which means that we can integrate near - field and far - field high - energy diffraction
microscopy methods and microtomography with in situ mechanical testing,» said Shade.
Thanks to emerging
microscopy methods, plaque has become a good source of traces of food that can now be analysed.
Because of the way light waves move through hyperbolic metamaterials, they can be used as superlenses to view objects too small to be seen with other
microscopy methods.
A new microscope merges different
microscopy methods to increase resolution and contrast in thick biological samples.
While working on his Ph.D., Dr. Taraska investigated the processes of triggered exocytosis and endocytosis in neuroendocrine cells with high - resolution
microscopy methods.
«Using different
microscopy methods, we were able to gain a better understanding of the phases, compositions and crystalline structures that allow these materials to convert light into electricity more efficiently,» said Poplawsky, adding that the availability of data is limited.
With different super-resolution
microscopy methods, they can even light up single molecules and their complex interactions with one another.
«We do not need to see down to the atomic scale, like many high - powered electron
microscopy methods offer,» Deb said.
The newly developed STED - RICS
microscopy method records rapid movements of molecules in live samples.
Researchers of Karlsruhe Institute of Technology (KIT) have developed a new fluorescence
microscopy method: STEDD (Stimulation Emission Double Depletion) nanoscopy produces images of highest resolution with suppressed background.
Scientists have developed and implemented a new
microscopy method that allows resolution of three - dimensional structures to 10 - 20 nm throughout entire cells, in samples up to 10 microns thick.
Researchers of Karlsruhe Institute of Technology have developed a new fluorescence
microscopy method: STEDD (Stimulation Emission Double Depletion) nanoscopy produces images of highest resolution with suppressed background.
From those measurements, the team made catalytic activity maps and used them along with
the microscopy method to selectively deposit tiny quantities of a cobalt borate OEC on the nanorod hot spots.
Not exact matches
Using a
method called two - photon
microscopy, they routinely measure the activity of hundreds of neurons with single cell resolution.
«Moving to non-invasive and repeatable
methods of nerve fiber measurements such as in vivo corneal confocal
microscopy would enhance study of peripheral neuropathy by enabling early detection of damage, progression of nerve fiber deterioration, and enable assessment of therapeutic strategies in the SIV / macaque model,» explains Dr. Mankowski.
The analysis of light spectrums after interactions with material with infrared spectroscopy and
microscopy allows the more precise and accurate conceptualization of research
methods.
The researchers used a number of
methods, including fluorescence and electron
microscopy, in collaboration with Dr. Eugenia Klein of the Institute's
Microscopy Unit; a unique system in Prof. Alon's lab for simulating blood vessels in a test tube; and in vivo imaging with Prof. Sussan Nourshargh of Queen Mary University of London.
The traditional
methods used in materials science analysis, like high - powered electron
microscopy and spectroscopy, do not combine chemical insights with the spatial resolution of IR imaging, the researchers said, so they can only provide average chemical measurements.
Because they are so small, multiple testing
methods are used, including cross-polarized light
microscopy, scanning electron
microscopy and PCR testing of the DNA of larvae in the water sample.
The researchers studied nanowires using X-ray
microscopy and with this
method they can pinpoint exactly how the nanowire should be designed to give the best properties.
On a study funded by the National Science Foundation and Agilent Technologies, researchers employed spatial light interference
microscopy (SLIM), a label - free
method, to perform localized measurements of light scattering in prostatectomy tissue microarrays.
Plowe is trying to develop surveillance
methods that hit the «sweet spot,» by which he means sensitive enough to detect very low parasite loads in asymptomatic carriers —
microscopy is too crude — but portable enough to use in the field.
Doctoral student Florian Vogel and Dr. Nelia Wanderka from the HZB Institute of Applied Materials have elegantly combined two
methods to accomplish this: transmission electron
microscopy (TEM) and atom probe tomography (APT), which they carried out in collaboration with colleagues from the University of Münster.
The research was reported in the journal Nature
Methods in a paper titled, «Ultrahigh - throughput single - molecule spectroscopy and spectrally resolved super-resolution
microscopy,» with co-authors Zhengyang Zhang, Samuel Kenny, Margaret Hauser, and Wan Li, all of UC Berkeley.
«New X-Ray
microscopy technique images nanoscale workings of rechargeable batteries:
Method could help researchers improve battery performance.»
To carry out the study, the team visualised a number of motors from different species of bacteria using a variant of a
method called cryo - election
microscopy, whose pioneers were awarded the Nobel Prize in Chemistry this year.
The reported femtosecond time resolution of four - dimensional (4D) electron
microscopy represents an advance of 10 orders of magnitude over that of conventional EELS
methods.
For this reason, the KIT researchers combined the RICS
method with STED
microscopy (stimulated emission depletion
microscopy).
«This
method allowed us to drastically expand the previously available data, acquired through scanning electron
microscopy; it also made possible to characterize surface patterns directly, not based upon analysis of metal replicas.
The new
method combines two types of
microscopy.
In order to track the movements of biological particles in a cell, scientists at Heidelberg University and the German Cancer Research Center have developed a powerful analysis
method for live cell
microscopy images.
Dr. Taraska's lab studies the structural cell biology of exocytosis and endocytosis with advanced imaging
methods including live cell
microscopy, superresolution fluorescence, and electron
microscopy.
Betzig and Moerner, working separately, developed a different
method known as single - molecule localization
microscopy.
In 1988, he became a principal investigator at AT&T Bell Laboratories where he extended his thesis work on near - field optical
microscopy, the first
method to break the diffraction barrier.
In Nature
Methods they describe how their software predicts the future cell type based on
microscopy images.
For this work, Goodell and colleagues including his collaborator Jody Jellison, now director of the Center for Agriculture, Food and the Environment at UMass Amherst, used a suite of investigative
methods including small angle neutron scattering (SANS), sum frequency generation (SFG) spectroscopy, Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), atomic force
microscopy (AFM) and transmission electron
microscopy (TEM) to fully describe the process.
The study examined the small - scale mechanical behaviour of teeth from limpets using atomic force
microscopy, a
method used to pull apart materials all the way down to the level of the atom.
Idrobo adds that new electron
microscopy techniques can complement existing
methods, such as x-ray spectroscopy and neutron scattering, that are the gold standard in studying magnetism but are limited in their spatial resolution.
A key component of the
method is two - photon
microscopy, used to generate a small point of light deep inside the sample.
Using a variety of
methods, including nuclear magnetic resonance spectroscopy, calorimetry and electron
microscopy, the researchers evaluated the fibers» structural and mechanical characteristics.
The research team is now exploring additional biological applications for the new system and is working to extend the
method to other microscope modalities, such as confocal
microscopy.
Neutron scattering is non-destructive, unlike electron
microscopy, another common
method used to investigate porous materials.
The research combined electron
microscopy, perfectly distilled proteins, and a
method of chemical freezing to isolate specific moments at the start of replication.