«It's a powerful example of what can be learned through combining state of the art
microscopy techniques with predictions from advanced computational approaches,» says James LeBeau, an assistant professor of materials science and engineering at NC State, co-author of the paper, and the creator of the revolving STEM technique.
Not exact matches
To investigate the nonlinear response of such plasmonic oligomers, the authors used a novel optical
microscopy technique, which is equipped
with polarization - structured beams.
The latest in live - cell
microscopy — multiphoton imaging, light - sheet
techniques, and technology borrowed from Raman spectroscopy — allow researchers to study living cells in more detail
with less effort.
Scientists would then be able to look at the slices
with microscopy and other
techniques to learn precisely what the brain looked like, right down to the cellular level.
In the lab, the team mixed each strain of K. veneficum
with a species of algae on which it preys, and recorded the three - dimensional motions of thousands of cells using a high - speed holographic
microscopy technique they described in 20071.
The
technique combines spectroscopy, which extracts information from interactions between light and matter,
with high - resolution
microscopy.
Mike Miller, a
microscopy expert at Oak Ridge National Laboratory in Tennessee is impressed
with the
technique.
A new nanopatterning
technique combines the advantages of near - field
microscopy with photolithography.
They then analyzed the shells
with electron
microscopy and other high - resolution imaging
techniques.
This is a close - up of the head region of the Alalcomenaeus fossil specimen
with the superimposed colors of a
microscopy technique revealing the distribution of chemical elements in the fossil.
The researchers have also been able to couple their new meso - level imaging
technique with extremely detailed electron
microscopy.
«Basically, this approach enables us to minimize the number of samples that need to be measured
with expensive
techniques such as atomic force
microscopy and transmission electron
microscopy,» Epps says.
Using electron
microscopy tools combined
with analytical spectroscopy
techniques, the researchers were able to examine the surface of bioprocessed Martian regolith simulants in detail.
This work,
with the assistance of soil scientists at the University of KwaZulu - Natal, has involved a suite of
techniques, including x-ray fluorescence (to provide quantitative data on minor and trace element composition), x-ray diffraction (to reveal crystal structure and parent rock types of paint ingredients), and environmental scanning electron
microscopy (to yield qualitative data on elements present).
Described in a study published in the journal Nature Communications, this novel approach uses high - speed atomic force
microscopy (AFM) combined
with a CRISPR - based chemical barcoding
technique to map DNA nearly as accurately as DNA sequencing while processing large sections of the genome at a much faster rate.
Much like in an old tube television where a beam of electrons moves over a phosphor screen to create images, the new
microscopy technique works by scanning a beam of electrons over a sample that has been coated
with specially engineered quantum dots.
«We studied a range of authentic microfossils using the same transmission electron
microscopy technique and in all cases these reveal coherent, rounded envelopes of carbon having dimensions consistent
with their origin from cell walls and sheaths.
In this role, she works
with many collaborators to facilitate implementing superresolution
microscopy into their research programs as well as developing novel
techniques for microbial live cell imaging.
The breakthrough came
with a new imaging
technique, dual - resonance - frequency - enhanced electrostatic force
microscopy (DREEM), which was developed by University of North Carolina at Chapel Hill chemist and co-author Dorothy Erie, former UNC and NC State postdoctoral researchers Dong Wu and Parminder Kaur, and was featured earlier this year in Molecular Cell.
The TSRI laboratories of Professor Erica Ollmann Saphire and Assistant Professor Andrew Ward are studying the structures of these antibodies using
techniques called electron
microscopy, which creates high - resolution images by hitting samples
with electrons, and X-ray crystallography, which determines the atomic structure of crystalline arrays of proteins.
Although SIM and other superresolution
techniques are pushing to spatial resolutions as high as 20 nanometers, they do not have the field of view of a
technique like Bessel beam
microscopy; they can only see a very small area, and imaging a larger area
with those
techniques takes a long time.
Both PALM and stimulated emission depletion (STED)
microscopy, the other super-resolution
technique recognized
with the 2014 Nobel Prize, illuminate samples
with so much light that fluorescently labeled proteins fade and the sample is quickly damaged, making prolonged imaging impossible.
Now, using this new
technique — which combines optical
microscopy with the use of particular organic compounds that make the energy of excitons visible — «we can directly say what kind of behavior the excitons were moving around
with.»
«It will show us how molecules behave, information that is not available from the micron - scale morphological images taken
with traditional
microscopy techniques.»
Generating videos of the nanowires stretching out required new methods to simultaneously label multiple features, keep a camera focused on the wriggling bacteria, and combine the optical
techniques with atomic force
microscopy to gain higher resolution.
Using confocal laser
microscopy and electron
microscopy, coupled
with the use of advanced synchrotron
techniques at the Swiss Light Source, in Villigen (Switzerland), and at the Canadian Light Source in Saskatoon, Canada, the team was able to show that not only the morphology of microbial twisted structures is preserved after long incubations under diagenetic conditions, but also organic remnants can be detected in the mineralized twisted structures.
Throughout, we established the performance advantages of lattice light - sheet
microscopy compared
with previous
techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.
Powerful new
microscopy techniques enable researchers to observe the whole process in living cells,
with bright fluorescent tags highlighting the chromosomes and other cellular components.
She and her research group recently demonstrated CLAIRE's imaging capabilities by applying the
technique to aluminum nanostructures and polymer films that could not have been directly imaged
with electron
microscopy.
Close - up of the head region of the Alalcomenaeus fossil specimen
with the superimposed colors of a
microscopy technique revealing the distribution of chemical elements in the fossil.
New
techniques combining various staining methods
with electron and light
microscopy make it possible to investigate in detail the connections among nerve cells and the circuitry of the brain
A new
technique enables 3 - D visualization of chromatin (DNA plus associated proteins) structure and organization within a cell nucleus (purple, bottom left) by painting the chromatin
with a metal cast and imaging it
with electron
microscopy (EM).
By parsing the cells
with slices of laser light and then correcting for any obstruction
with the same AO
technique astronomers use to correct blurriness in observations of stars, the scientists have come up
with a
microscopy technique that looks like an artistic rendering.
Another
technique pinpoints the locations of proteins within their subcellular context by coupling super-resolution imaging
with electron
microscopy.
With cutting - edge crystallography and
microscopy techniques, Christopher Barnes aims to reveal — in extreme detail — how newly isolated antibodies neutralize HIV - 1 by latching onto viral envelope proteins.
Researchers aim to answer these questions using cutting - edge electron
microscopy techniques in combination
with molecular and biochemical approaches.
Scientists have combined robotic
techniques with atomic force
microscopy to achieve understanding of how skin falls apart at the nanoscale.
The developed
microscopy approach combines two fluorescence
microscopy techniques: REversible Saturable OpticaL Fluorescence Transitions (RESOLFT)
Microscopy with reversible switchable fluorescent proteins (RSFPs) and 4Pi -
Microscopy.
This
technique is faster and provides a wider field of view than more traditional 3D
techniques such as scanning electron
microscopy combined
with electron energy - loss spectrometry or atom probe tomography.
She uses a new ultra-fast
microscopy technique to record the activity in the whole fly brain and works closely
with theoretical neuroscientists to analyze the data and model network activity.
The team saw the oxygen atoms flow out of the cerium oxide clusters and slowly spread farther and farther away by combining high - resolution scanning tunneling
microscopy with a conventional surface - sensitive
technique, called Auger spectroscopy.
For this latest study of DNA nanostructures, Ren used an electron - beam study
technique called cryo - electron
microscopy (cryo - EM) to examine frozen DNA - nanogold samples, and used IPET to reconstruct 3 - D images from samples stained
with heavy metal salts.
The combination of NLO
microscopy techniques providing morphological information, together
with those providing functional information is a crucial issue for tissue classification and pathological assessment.
To celebrate the 30th anniversary of the Nobel Prize in scanning tunnelling
microscopy (STM) and the 30th anniversary since the first paper in atomic force
microscopy (AFM), Nanotechnology ™ has been organising a focus collection
with guest editors Franz Giessibl, Rodolfo Miranda and Johannes Barthes to collate some of the latest cutting - edge progress developing and exploiting these scanning probe
techniques.
Correlative light and electron
microscopy (CLEM) is an imaging
technique that combines electron
microscopy (EM)
with fluorescence localization data.
Acoustic emission measurements collected during electrochemical tests, combined
with advanced imaging
techniques such as transmission X ‐ ray
microscopy, provide a window into the internal workings of battery materials during energy storage cycles.
They also assist
with more advanced
techniques, such as live - cell
microscopy, three - dimensional reconstruction, and image analysis.
Acoustic emission measurements collected during electrochemical tests, combined
with advanced imaging
techniques such as transmission X ‐ ray
microscopy, provide a window into the internal workings of battery materials...
The journal also focuses on the technological aspects of pathology, including molecular biology
techniques, morphometry aspects (stereology, DNA analysis, syntactic structure analysis), communication aspects (telecommunication, virtual
microscopy, virtual pathology institutions, etc.), and electronic education and quality assurance (for example interactive publication, on - line references
with automated updating, etc.).
Caroline obtained her degree in physics from the University of Heidelberg, Germany in 2008 and went on to do a PhD at the Institute of Photonics, University of Strathclyde, Glasgow in 2012
with a thesis on the application of wavefront sensorless adaptive optics in advanced
microscopy techniques.