Introduce your students to
microscopy with my fun «what's under the microscope?»
Transmission electron
microscopy with colloidal gold immunocytochemistry showed structural damage in young and aged microvessel endothelium of ApoE4 animals extended to the cytoplasm of perivascular cells, perivascular nerve terminals and hippocampal neurons and glial cells.
The course is an introduction to both light and electron
microscopy with solid theoretical background extended with many practical presentations.
We propose to develop a novel
microscopy with super-resolution capability to «zoom into» these dots.
We also determined the positions of various nuclear pore complex (NPC) components and directly resolved the ring - like structure of the NPC, combining stochastic super-resolution
microscopy with single particle averaging.
O.S. did transmission electron
microscopy with T.B. and A.L. P.R.H.S. cloned and characterized twist.
Images were analyzed by LSM700 confocal microscopy (Zeiss) or DM5000B fluorescence
microscopy with a DFC500 CCD camera (Leica).
Methods: The team's method combines high - angle annular dark - field scanning transmission electron
microscopy with X-ray energy dispersive spectrometry.
It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning
microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.
The team saw the oxygen atoms flow out of the cerium oxide clusters and slowly spread farther and farther away by combining high - resolution scanning tunneling
microscopy with a conventional surface - sensitive technique, called Auger spectroscopy.
The paper is titled «Motionless volumetric photoacoustic
microscopy with spatially invariant resolution.»
Using scanning capacitance
microscopy with a Park Systems atomic force microscope a team at NASA successfully characterized both the spatial variations in capacitance as...
I was combining cryo - fluorescence
microscopy with cryo - electron tomography.
One of the main tools to gather insights on these questions is
microscopy with a heavy focus on live imaging.
All sections were viewed under epiflourescence
microscopy with an FITC - specific filter block (Nikon UK Ltd, Surrey, UK) and images recorded using an integrating camera (Model JVC KY - SSSB: Foster Findlay and Associates, Newcastle upon Tyne, UK).
The researchers used high - resolution scanning electron
microscopy with energy dispersive x-ray analysis to study microstructure and elemental composition, and high - resolution x-ray photoelectron spectroscopy for more detailed chemical characterization.
By using video
microscopy with fluorescent tagging of the two organelles, the scientists observed that the mitochondria and lysosomes formed stable contacts inside living human cells.
The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light
microscopy with electron microscopy.
By combining scanning electron
microscopy with x-ray computer tomography to examine the bead's microstructure and chemistry, a British research team determined that the iron possessed a structural and microchemical signature known as the Widmanstätten pattern that is unique to weathered iron meteorites.
Now, using this new technique — which combines optical
microscopy with the use of particular organic compounds that make the energy of excitons visible — «we can directly say what kind of behavior the excitons were moving around with.»
This procedure is similar to the conventional optical
microscopy with the picture showing the distribution of local magnetic fields.
Researchers at Columbia University have reported a new approach to visualize glucose uptake activity in single living cells by light
microscopy with minimum disturbance.
A new nanopatterning technique combines the advantages of near - field
microscopy with photolithography.
The image was taken with multiphoton
microscopy with wavelength mixing, and it received an Honorable Mention in the 2012 Olympus BioScapes Digital Imaging Competition.
Dunsby's goal was to perform light - sheet
microscopy with a single objective, while maintaining good resolution.
A European lab combines «light sheet»
microscopy with an illumination process that subtracts the static caused by scattered photons to devise a way to clearly observe the inner workings of cells over a period of days
Not exact matches
To investigate the nonlinear response of such plasmonic oligomers, the authors used a novel optical
microscopy technique, which is equipped
with polarization - structured beams.
Like beacons in a dark sea, these cells glowed
with evidence of a norovirus infection in fluorescent
microscopy images, researchers report in the April 13 Science.
Dr Ohmann's colleagues from Soochow University calculated atomic structures using principles of quantum physics and then compared them
with scanning tunneling
microscopy data.
Using a method called two - photon
microscopy, they routinely measure the activity of hundreds of neurons
with single cell resolution.
While light - sheet
microscopy is an old idea — scientists at ZEISS
Microscopy and collaborators first came up
with it in 1903 — only in this century has the convergence of fluorescent labels that work to process image volumes combined to make light - sheet mainstream.
The kit features true multiplexing capabilities
with green fluorescent protein (GFP) and other green fluorescent probes, and is optimized for both fluorescence
microscopy and flow cytometry applications.
Several physical measurements, including electron
microscopy, assured us that our product was a closed loop coiled tightly around the virus - DNA template and that it was identical in size and other details
with the replicative form of DNA that appears in the infected cells.
The latest in live - cell
microscopy — multiphoton imaging, light - sheet techniques, and technology borrowed from Raman spectroscopy — allow researchers to study living cells in more detail
with less effort.
Efficient collection and detection of fluorescence coupled
with careful minimization of background from impurities and Raman scattering now enable routine optical
microscopy and study of single molecules in complex condensed matter environments.
«We believe that our approach is broadly applicable to other low - cost
microscopy systems that use, for example, inexpensive lenses or cameras, and could facilitate the replacement of high - end bench - top microscopes
with cost - effective, mobile alternatives.»
These include more sensitive mass spectrometry for detecting, identifying and quantifying protein molecules, electron
microscopy for visualizing an assortment of components of the nano - machine that transports toxins, and fluorescence confocal
microscopy, which can label proteins
with light emitting dyes.
We conclude
with current explorations in imaging of nanomaterials and biostructures and an outlook on possible future directions in space - time, 4D electron
microscopy.
The stretching of single, tethered DNA molecules by a flow was directly visualized
with fluorescence
microscopy.
Individual carbocyanine dye molecules in a sub-monolayer spread have been imaged
with near - field scanning optical
microscopy.
Five years ago, using a lower - resolution form of electron
microscopy in which the protein is visualized in the presence of negative stain, Whelan's team was able to detect at low resolution a structure that looked like a doughnut
with three globular domains.
In near - field scanning optical
microscopy, a light source or detector
with dimensions less than the wavelength (λ) is placed in close proximity (λ / 50) to a sample to generate images
with resolution better than the diffraction limit.
The analysis of light spectrums after interactions
with material
with infrared spectroscopy and
microscopy allows the more precise and accurate conceptualization of research methods.
We determined the cryo — electron
microscopy (cryo - EM) structures of HMAb 2D22 complexed
with two different DENV2 strains.
Fluorescently labeled synaptic vesicles inside the axons of cultured neurons were recorded
with stimulated emission depletion (STED)
microscopy in a 2.5 - micrometer by 1.8 - micrometer field of view.
The new 3 - D structure, which was obtained using X-ray crystallography and electron
microscopy, revealed that the outer surface of the Zika NS1 protein has substantially different electrical - charge properties than those of other flaviviruses — indicating it may interact differently
with the members of an infected person's immune system.
The group then ran dozens of
microscopy tests
with Dan Wagner, Rice associate professor of biochemistry and cell biology, to see how different formulations and amounts of biochar affected cell signaling.
Scientists would then be able to look at the slices
with microscopy and other techniques to learn precisely what the brain looked like, right down to the cellular level.
The electron
microscopy provided a crucial piece of the larger puzzle assembled in concert
with Berkeley Lab materials scientists and soft x-ray spectroscopy experiments conducted at SLAC's Stanford Synchrotron Radiation Lightsource (SSRL).
The researchers used a number of methods, including fluorescence and electron
microscopy, in collaboration
with Dr. Eugenia Klein of the Institute's
Microscopy Unit; a unique system in Prof. Alon's lab for simulating blood vessels in a test tube; and in vivo imaging
with Prof. Sussan Nourshargh of Queen Mary University of London.