Sentences with word «microtiter»

To determine whether HPCs stimulate alloreactive T cells, 5 × 105 129SvJ CD45 + HPCs, splenocytes or bone marrow cells were irradiated with 20 cGy and plated into 96 - well microtiter plates in RPMI medium supplemented with 10 % FCS, gentamycin and streptomycin.

The proliferative activity was determined every 24 h by the 3 -(4,5 - dimethylthiazol -2-yl)-2,5-diphenol-tetrazolium bromide assay using a Dynatech MR - 5000 96 - well microtiter plate reader set at 570 nm.

Microplate readers are used to detect biological, chemical or physical events in microtiter plates via the measurement of light.

Briefly, recombinant proteins were coated overnight on microtiter plates at room temperature in equimolar amounts.

These include 5 high performance cell sorters that are customized for isolating cells and chromosomes at high speed and 4 high throughput cell analysers that maximize the speed of acquisition of specimens from microtiter well plates (96 well plate, 384 well plate).

CBCS facilities comprise compound handling labs (hosting the SciLifeLab compound collection of ca 200,000 molecules), screening labs (microtiter format liquid handling robotics and a wide range of plate readers), and chemistry labs (equipment and instrumentation for organic chemistry synthesis and analysis).

The kits use a rapid, one - step protocol, employing preblocked microtiter well strips coated with biotin.

Unique microtiter plate enzyme immunoassay for the quantitation of virions and assembled empty capsids of human Adeno - associated Virus 5.

Conventional screens are based on microtiter plates and depend on the use of robots, but cost has been a major limiting factor in scaling its use.

Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.

Using a standard inverted microscope, researchers can then lift the desired cells and transfer them into individual sample tubes or microtiter wells.

The Eukaryotic Single - Cell Analysis facility will be placed in Stockholm and will offer single - cell RNA - seq on the Fluidigm C1 platform using the STRT protocol, as well as in a microtiter - plate format using SMART - seq2.

On the Persomics ImagineArray ™ platform, microtiter wells are replaced with contact - printed spots.

After 5 minutes of incubation at room temperature in the dark, ViaCount / cell mixture was loaded into a 96 - well microtiter plate and read by the Guava Flow Cytometer.

Microtiter plates were coated with 100 µl / well BYD virus antigen at a concentration of 6 µg / ml in carbonate / bicarbonate buffer (pH 9.6) overnight at 4 °C.

«The problem is that the wells on a microtiter plate have spatial information.