Sentences with phrase «microtiter plate»
A microtiter plate is a tool used in laboratories to conduct experiments and tests. It is a small flat plate with multiple wells or indentations where tiny amounts of liquids, like samples or reagents, can be placed. These wells are organized in a grid pattern, making it easy for scientists to analyze and compare different liquids simultaneously. Full definition
To determine whether HPCs stimulate alloreactive T cells, 5 × 105 129SvJ CD45 + HPCs, splenocytes or bone marrow cells were irradiated with 20 cGy and plated into 96 - well microtiter plates in RPMI medium supplemented with 10 % FCS, gentamycin and streptomycin.
journals.plos.org
Microplate readers are used to detect biological, chemical or physical events in microtiter plates via the measurement of light.
Briefly, recombinant proteins were coated overnight on microtiter plates at room temperature in equimolar amounts.
The proliferative activity was determined every 24 h by the 3 -(4,5 - dimethylthiazol -2-yl)-2,5-diphenol-tetrazolium bromide assay using a Dynatech MR - 5000 96 - well microtiter plate reader set at 570 nm.
Unique microtiter plate enzyme immunoassay for the quantitation of virions and assembled empty capsids of human Adeno - associated Virus 5.
Conventional screens are based on microtiter plates and depend on the use of robots, but cost has been a major limiting factor in scaling its use.
Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.
After 5 minutes of incubation at room temperature in the dark, ViaCount / cell mixture was loaded into a 96 - well microtiter plate and read by the Guava Flow Cytometer.
Microtiter plates were coated with 100 µl / well BYD virus antigen at a concentration of 6 µg / ml in carbonate / bicarbonate buffer (pH 9.6) overnight at 4 °C.
«The problem is that the wells on a microtiter plate have spatial information.