Sentences with word «microtome»

A "microtome" is a tool or device used in science and medicine to cut extremely thin slices of a material, such as tissue samples, for examination under a microscope. Full definition
Sections are cut at 40 µm thick using a freezing sliding microtome.
But maybe quantitative analysis of a few select microtomed sections would help.
After the brains equilibrated in sucrose, they were cut into 40 μm coronal sections with a freezing microtome (Leica SM 2000R, Germany).
The control of biological material must have been a bit more lax in the late 1970s (maybe cautiousness inversely correlates with lapel width), because she was somehow able to sneak a piece of my (our) umbilical cord to her lab microtome, where she carved two thin sections and mounted them on glass slides, just for fun.
Twenty - two sections of paraffin - embedded tissue were stained for Luxol fast blue, hematoxylin and eosin, Bielschowsky silver, phosphorylated tau (ptau)(AT8), α - synuclein, amyloid - β, and phosphorylated transactive response DNA binding protein 43 kDa (pTDP - 43) using methods described previously.16 In some cases, large coronal slabs of the cerebral hemispheres were also cut at 50 μm on a sledge microtome and stained as free - floating sections using AT8 or CP - 13.16,17
Authored SOP and developed techniques for Fuchsin staining, microtome prepping, and analyzing biopsies.
· Change hemotoxin and eosin staining machine chemicals · Coverslip and prepare slides for the doctor · Cut specimen on microtome · Gross dermatology specimen · Embed processed blocks in...
Brains were fixed with 4 % paraformaldehyde in 100 mM sodium tetraborate, pH 9.5, for 3 h, cryoprotected with 20 % sucrose - potassium - PBS (KPBS), and sectioned into coronal (30 μm) sections using a sliding microtome (Leica Microsystems Inc, Buffalo Grove, IL, USA).
Tissue was cryoprotected in 30 % Sucrose dissolved in PBS for 24 h and cut on a freezing microtome (Leica 1850, Solms, Germany) in six series of 40 µm thick sections in either the frontal or the sagittal plane.
The Slice Once the gelatin sets, the brain is frozen, mounted on a motorized tissue - slicing device called a microtome, and sliced.
In his spare time, Purkinje improved techniques for preparing tissue samples, using a microtome — an instrument that slices tissues into very thin samples.
«They told me it was not for the girls,» she recalls, «that it was only for the boys because you need to know physics and optics... I showed them that I could be completely self - sufficient: prepare my samples, use the microtome, make the examination.
Fetal thoraces and adult hearts and aortas were embedded in paraffin, exhaustively sectioned (10 µm, Leica RM 2235 microtome, Germany) and processed for haematoxylin and eosin (H&E) staining.
Frozen tissue samples were sectioned at 16μm with a microtome cryostat (Cryostar HM 560 MV; Microm International GmbH) and placed on slides that were stored at -20 °C.
Brains were cut into 30 µm coronal sections on a sliding microtome (Leica) and the free - floating sections were used for immunostaining.
Each microtome is positioned in a separate room with adequate bench space and ancillary specimen preparation equipment so as to be self - contained sectioning areas
X-gal stained, paraformaldehyde fixed embryos were embedded in paraffin wax and sectioned transversely in a microtome at 7 micron intervals.
Maintains all equipment, cryostat, microtomes, embedding station, all Venatan stainers, in order to ensure accurate and appropriate performance.
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