Sentences with phrase «microtomed sections»

Slides with microtome sections (10 mm thick) of wild - type and bsl1 - 1 mutant developing inflorescences were deparaffinized and treated with 5 μg / mL proteinase K at 37 °C for 20 min followed by refixation in PFA solution (4 % [w / v] paraformaldehyde, 5 % [v / v] acetic acid, and 50 % [v / v] ethanol) for 10 min.

Not exact matches

The control of biological material must have been a bit more lax in the late 1970s (maybe cautiousness inversely correlates with lapel width), because she was somehow able to sneak a piece of my (our) umbilical cord to her lab microtome, where she carved two thin sections and mounted them on glass slides, just for fun.

Fetal thoraces and adult hearts and aortas were embedded in paraffin, exhaustively sectioned (10 µm, Leica RM 2235 microtome, Germany) and processed for haematoxylin and eosin (H&E) staining.

Brains were fixed with 4 % paraformaldehyde in 100 mM sodium tetraborate, pH 9.5, for 3 h, cryoprotected with 20 % sucrose - potassium - PBS (KPBS), and sectioned into coronal (30 μm) sections using a sliding microtome (Leica Microsystems Inc, Buffalo Grove, IL, USA).

Sections are cut at 40 µm thick using a freezing sliding microtome.

Brains were cut into 30 µm coronal sections on a sliding microtome (Leica) and the free - floating sections were used for immunostaining.

Each microtome is positioned in a separate room with adequate bench space and ancillary specimen preparation equipment so as to be self - contained sectioning areas

X-gal stained, paraformaldehyde fixed embryos were embedded in paraffin wax and sectioned transversely in a microtome at 7 micron intervals.

Remaining prostate tissue was formalin - fixed and paraffin - embedded (FFPE), sectioned with a microtome and placed on microscope slides for FISH analysis (referred to as FFPE sections).

Twenty - two sections of paraffin - embedded tissue were stained for Luxol fast blue, hematoxylin and eosin, Bielschowsky silver, phosphorylated tau (ptau)(AT8), α - synuclein, amyloid - β, and phosphorylated transactive response DNA binding protein 43 kDa (pTDP - 43) using methods described previously.16 In some cases, large coronal slabs of the cerebral hemispheres were also cut at 50 μm on a sledge microtome and stained as free - floating sections using AT8 or CP - 13.16,17