Sentences with phrase «midgut contents»
Confocal microscopy confirmed that spirochetes were present within the salivary glands and did not represent surface contamination by midgut contents during dissection (Fig. 5B).
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In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).
The tick midgut contents were also subjected to RT - PCR.
A portion of the XT midgut contents were also placed in culture.