Sentences with phrase «midgut of»

Here, Marianes and Spradling have investigated a type of adult stem cell, known as intestinal stem cells, that resides in the midgut of fruit flies.

Results: A total of 21,427 ESTs were produced from the midgut of adult Glossina morsitans morsitans and grouped into 8,876 clusters or singletons potentially representing unique genes.

Plasmodium ookinetes and sporozoites have to travel from the midgut of the mosquito into the salivary gland to be transmitted to a human host.

The external cuticle on the ventral surface is analogous to the peritrophic membrane (found in the midgut of other insects) in adult females (Kathirithamby 2000).

Glycosidases in the midgut of the tobacco hornworm larvae remove only one sugar molecule from lyciumoside IV which converts the toxin into a novel compound.

«Biochemical networks mapped in midgut of Aedes aegypti mosquitoes.»

W. R. Harvey, S. Nedergaard, Sodium - independent active transport of potassium in the isolated midgut of the Cecropia silkworm.

Not exact matches

First, the lining of mosquito midgut (equivalent of stomach and small intestine) has properties that can prevent the pathogen or parasite from getting out of the gut.

When food exits the pouch, it shoots straight into her midgut, continuing largely undigested until it reaches the Y junction and combines with the acidic brew of stomach juices.

Mario Sobern of the National Autonomous University of Mexico and his colleagues investigated the most commonly used Bt toxins, which work by binding to specific receptors in pest midguts.

Malaria parasite transmission depends on the successful transition of Plasmodium through discrete developmental stages in the lumen of the mosquito midgut.

Indy was most abundantly expressed in the fat body, midgut, and oenocytes: the principal sites of intermediary metabolism in the fly.

Mcf1 treated caterpillars show rapid loss of body turgor (the «floppy» phenotype) within 12 hours, associated with massive apoptosis of the midgut epithelium.

Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome — Laura B. Dickson — BMC Parasites & Vectors

Nonautonomous regulation of Drosophila midgut stem cell proliferation by the insulin - signaling pathway.

To probe alternative modes of ISC replacement, Ohlstein and colleagues developed a physiologically - relevant starvation assay that elicits a rapid and severe loss of ISCs in the Drosophila posterior midgut.

A portion of the XT midgut contents were also placed in culture.

Given that our wild type B. burgdorferi acquired by xenodiagnostic ticks retain the ability to express ospC (also shown by RT - PCR in Fig 4), we can not rule out the possibility that a tick midgut - adapted phenotype, in the absence of salivation and feeding, contributed to the failure of the B. burgdorferi acquired from XT to infect SCID mice.

Panel A = positive control for IFA using B. burgdorferi culture; Panel B = XT from animal IH11 (treated); Panel C = XT from animal IK14 (treated); Panel D = XT from animal IL09 (treated); Panel E = positive control for DFA using midgut smear of tick that was capillary tube - fed B. burgdorferi; Panel F = XT from animal IP55 (untreated); Panel G = XT culture pellet from animal IP55 (untreated); Panel H = XT culture pellet from animal IK14 (treated).

Here, tick midguts were pooled per animal, pelleted, washed with 5 mL HBSS and then resuspended in HBSS in a final volume of 0.25 mL for mouse injections.

Finally, stomodeal - specific RNAi - mediated silencing of Twist during compression impairs the differentiation of midgut cells, resulting in larval lethality.

In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).

(A) IFA of midgut tissues from partially fed nymphal ticks infected with B31 - A3 WT, ospC7 mutant, or ospC7 / ospC +4 complemented B. burgdorferi clones.

Aspects of the midgut environment that change during tick feeding, such as temperature, pH, and nutrients, influence the expression of many B. burgdorferi genes, including ospC (3, 9 - 11).

IFA analysis of midguts from a subset of ticks immediately after immersion demonstrated that a small number of spirochetes from all three strains had been ingested.

Larval ticks from all three groups contained increased numbers of spirochetes, suggesting that the ospC mutant, in addition to the WT and complemented clones, could infect and replicate in the tick midgut.

Spirochetes begin to synthesize OspC during the tick blood meal, while they are still in the tick midgut, and the expression of ospC is subsequently down - regulated after transmission of B. burgdorferi to the mammal (3, 26, 27).

In this model, OspA is proposed to be an adhesin that tethers the spirochetes to the midgut epithelium (5, 6), whereas OspC is thought to be important for movement of the spirochete within the tick (7, 8).

Unfed and fed nymphs from all three groups retained midgut infections of spirochetes (Fig. 5A).

We have undertaken an expressed sequence tag (EST) project on the adult tsetse midgut, the major organ system for establishment and early development of trypanosomes.

Our experiments significantly expand previous knowledge of regional variation within the Drosophila midgut.

For example, intestinal stem cells (ISCs) of the adult Drosophila midgut (Ohlstein and Spradling, 2006; Micchelli and Perrimon, 2006) generate the two major differentiated cell types of the gut, enterocytes and enteroendocrine cells, throughout life.

Credit for the discovery of detailed midgut regionalization deserves to be shared equally between the two groups.