Salk scientists and colleagues have proposed new molecular criteria for judging just how close any line of laboratory - generated stem cells comes to
mimicking embryonic cells seen in the very earliest stages of human development, known as naïve stem cells.
Not exact matches
Now, a collaborative team of scientists at Washington University in St. Louis has developed a new process to generate NP - like
cells from hiPSCs, one that truly goes back to the beginning and
mimics the process of
embryonic development.
Yamanaka's group and two others followed up earlier this year with firmer evidence that these induced pluripotent stem (iPS)
cells faithfully
mimicked the patterns of gene activity and cellular differentiation observed in
embryonic stem
cells.
Instead of
mimicking the complex 3D organization of the developing pituitary gland, this approach relies on the precisely timed exposure of human pluripotent stem
cells to a few specific cellular signals that are known to play an important role during
embryonic development.
When current methods for generating naïve stem
cells in the lab were judged using the three tests, each fell short of
mimicking the naïve
embryonic cells in different ways.
They then exposed these
cells to certain growth factors in - vitro to cause them to turn into liver - like
cells, in a process that
mimics embryonic development.
Now researchers have found a way to create stem
cells that
mimic the universal role of
embryonic cells.
This reprogramming methodology dispenses with the standard nucleic acid - mediated strategy and instead treats
cells with conditioned media (CM) designed to
mimic the primitive
embryonic environment.
One is the molecular identification of
cell growth and differentiation factors, and their receptors that
mimic embryonic developmental cues, and allow PSCs to differentiate into almost any mammalian
cell type.
By: Sadhana Agarwal, Katherine L. Holton, Robert Lanza Differentiation of human
embryonic stem
cells (hESCs) to specific functional
cell types can be achieved using methods that
mimic in vivo
embryonic developmental programs.
Since human
embryonic stem
cells grow in an adherent culture system, for
cells being reprogrammed this «new culture system» is an adherent culture system (to try and
mimic the conditions the
embryonic stem
cells want to be happy).
But the eyebrow - raising reports claimed that adult stem
cells sometimes behave like their
embryonic counterparts,
mimicking their trademark capacity to engender all types of
cells — an ability dubbed pluripotency.
Researchers announced they
mimicked the way
embryonic stem
cells develop into heart muscle in a lab.
Virginia Commonwealth University (VCU), Richmond • VA 2008 — 2008 Experiment Design, Project Innovatively designed an experiment that would utilize
embryonic stem
cells, and scaffold to
mimic kidney function.