Sentences with phrase «minimum essential medium»

Minimum essential medium alpha (MEMα), MEM, Dulbecco's modified Eagle medium / nutrient mixture F12 (DMEM / F12), and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY).

Mouse iPS cells were maintained with 90 % the Glasgow Minimum Essential Medium (GMEM, Invitrogen) containing 10 % FBS, 100 U / ml penicillin, 100 µg / ml streptomycin, 0.1 mM β - mercaptoethanol and 2 mM L - glutamine.

Vero E6 tissue cultures [obtained from The American Type Culture Collection (ATCC), CRL: 1586] were grown in Dulbecco's modified minimum essential medium (DMEM) supplemented with penicillin (100 units / ml), streptomycin (100 µg / ml), 0.2 % sodium bicarbonate and 10 % fetal bovine serum (FBS).

MDA - MB 231 and BCM2 cell lines were cultured in minimum essential medium (MEM)(Invitrogen Corporation, Carlsbad, CA, USA) supplemented with 10 % fetal bovine serum (HyClone, Logan, UT, USA; Thermo Fisher Scientific Inc., Waltham, MA, USA), 2 mM L - glutamine, 1 mM sodium pyruvate, 1 mM non-essential amino acids, and 1 % vitamin (HyClone).

For virus isolation attempts, serum samples were diluted 1 ∶ 5 in Eagle's minimum essential medium (EMEM) supplemented with 2 % fetal bovine serum, 200 µg of streptomycin, and 200U / ml of penicillin.

Geniculate ganglia were rapidly excised from CO2 - euthanized mice and placed in enzyme solution [minimum essential medium with Earle's balanced salts (MEM / EBSS; Hyclone) containing 1.25 mg / ml trypsin (Sigma - Aldrich) and 2.5 mg / ml collagenase A (Roche Diagnostics)-RSB- for 30 min.