Therefore, Teitell's group opted to further modify their constructs with a 3 ′ - UTR mitochondrial targeting sequence (MTS) from human
mitochondrial ribosomal protein S12 — the same essential approach used to optimize the import of allotopically - expressed proteins by Dr. Marisol Corral - Debrinski, (3,4) and subsequently advanced for multiple additional ETS subunit proteins by Dr. Matthew O'Connor's group at the SENS Foundation Research Center (RC).
The branch uniting the fungi and animals is well - supported based on a number of molecular phylogenetic datasets, including the nuclear small subunit
ribosomal RNA gene (Wainwright et al., 1993; Bruns et al. 1993), unique and shared sequence insertions in
proteins such as elongation factor 1α (Baldauf and Palmer, 1993), entire
mitochondrial genomes (Lang et al., 2002), and concatenated
protein - coding genes (Steenkamp et al., 2006).