Sentences with phrase «mixed lymphocyte»

One of the assays widely used in the literature is the application of splenocytes from tolerant animals as responder cells in a 4 - day mixed lymphocyte culture.

Based on the low immunogenicity of the HPCs in the mixed lymphocyte cultures, we now wondered whether the HPCs would engraft in allogenic recipients.

In addition, mixed lymphocyte cultures using the cells of the chimeric animals as responder cells to donor splenocytes failed to demonstrate any sensitization or clonal deletion of alloreactive T cells; i.e. T cell responses were identical to those of control animals (n = 4)(data not shown).

Additionally, splenocytes of recipient animals were used as responder cells in mixed lymphocyte reactions and ELISPOT to determine responses to donor alloantigen.

Cellular biology: Isolation and culture of various cell types; cryopreservation; Mixed Lymphocyte Reaction (MLR); cloning by limiting dilution; cytotoxicity assays using Chromium - 51 release; proliferation assay (CFSE and 3H - thymidine); ELISA; Elispot; magnetic beads separation.

Using a mixed lymphocyte population obtained from a mucosal tissue (tonsils) we treated cells with or without lethal toxin for 3 hr and then stimulated the cells with or without IL - 23.

Abbreviations: AD - MSC: adipose tissue derived mesenchymal stem cells; APC: antigen presenting cell; BM: bone marrow; CNI: calcineurin inhibitor; DC: dendritic cells; GVHD: graft - versus - host disease; HLA: histocompatibility locus antigen; HGF: hepatocyte growth factor; IL: interleukin; IDO: indoleamine 2,3 - dioxygenase; MLR: mixed lymphocyte reaction; MSC: mesenchymal stem cells; PGE2: prostaglandin E2; SC: stem cells; TGF: transforming growth factor; TH: T - helper; T - regs: T - regulatory cells.

Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex.

Not exact matches

However, for the NOS group the reverse was seen, with overcrowding being associated with an increased incidence of this type of HL, while deprivation seemed to have no effect on the incidence of the mixed cellularity and lymphocyte - rich sub-types.

The researchers found five different sub-types of HL among the patients studied: 247 cases of the nodular sclerosis (NS) type, in which the tumour nodules are large; 105 of mixed cellularity, where a mixture of different types of inflammatory cells are involved; 58 lymphocyte rich, the sub-type with the best outcome; 68 «others»; and 143 «not otherwise specified» (NOS).

Many leukemias exhibit mixed - lineage patterns of gene expression, for example of both macrophage and lymphocyte genes.

Histopathological analysis of rejected corneal grafts in both human and animal subjects reveals the presence of a mixed inflammatory infiltrate containing both CD4 + and CD8 + T lymphocytes (4 — 7).

«It's been a puzzle that lots of leukemias exhibit mixed lineage patterns of gene expression - for example of both macrophage and lymphocyte genes,» he said.