Sentences with phrase «ml cell lysis»

Cell pellets were resuspended in 1 ml cell lysis buffer [5 mM Pipes pH 8.0, 85 mM KCl, 0.5 % NP - 40] containing protease inhibitors and incubated for 10 min on ice.

Not exact matches

Reactions were terminated with 1 ml of ice - cold PBS, and cells were centrifuged and resuspended in 50 μl of cell lysis buffer containing 1 % NP - 40 and protease inhibitors.

Make up «direct lysis buffer» by adding 1 mL of Proteinase K (e.g. Sigma P4850) to 100 mL DirectPCR Lysis Reagent (cell)(Viagen Biotech, Cat # 302 lysis buffer» by adding 1 mL of Proteinase K (e.g. Sigma P4850) to 100 mL DirectPCR Lysis Reagent (cell)(Viagen Biotech, Cat # 302 Lysis Reagent (cell)(Viagen Biotech, Cat # 302 - C).