Sentences with phrase «ml concentration»

Alternatively, use the dropper bottle: 2 drops for each lb body weight for the 0.5 mg / mL concentration (5 drops for each kilogram of body weight).

I am assuming that the testosterone you are using is a standard 200 mg / ml concentration.

Not exact matches

Its average concentration in milk is 0.01 g / 100 ml but lactoferrin is found in higher concentration in whey protein products: 30 to 100 mg / l of sweet whey.

The overall contribution of sugars in these products to the food supply was determined by multiplying volume sales per annum by the concentration of sugars per 100 mL for each category of beverage.

The new infant drops have the same concentration as Children's Tylenol ® (160 mg / 5 ml per dose).

Serum cotinine, a metabolite of nicotine, was used to classify participants as exposed to ETS when its concentration was between 0.015 mg / ml (the lower detection limit of the assay) and 3ng / ml.

The participants were offered genetic testing in cases where sperm concentrations fell below 5 million / ml, and they were offered written feedback, appropriate counselling, investigation and follow - up.

This tiny biochip can sensitively detect proteins and nano - sized polymer vesicles with a concentration as low as 10ng / mL (150 pM) and 3.75 μg / mL respectively.

Summer blood concentrations of vitamin D were higher for both groups: 27 ng / ml in rural individuals and 39 ng / ml in the urban dwellers.

Overall, the smartphone - based device was able to detect abnormal semen samples based on WHO thresholds on sperm concentration and motility (sperm concentration < 15million sperm / ml and / or sperm motility < 40 %) with an accuracy of 98 percent.

The formulation is not concentrated, as naloxone syringes are only commercially available in concentrations of up to 1mg / ml.

NASA's upper atmosphere research satellite, for example, carries the British - made microwave limb sounder (MLS) which measures concentrations of chlorine monoxide — a chemical that plays a critical part in the destruction of ozone.

Of the 898 high risk women followed in the study, only 3.8 % of women with concentrations less than 10 ng / mL (tested at 18 - 21 weeks) delivered before 34 weeks of gestation, a similar rate to that expected in a normal pregnancy.

IAPP solution in PBS, after overnight aggregation, was centrifuged and resuspended to an estimated concentration of 5 mg / ml.

To ascertain whether the hemocyte paralysis effect of Mcf1 occurs in a dose - dependent manner we injected embryos with half the concentration previously used (0.1 mg / ml).

To determine limits of sensitivity for the assay, probit analysis of results from 6 PCR replicates of 7 serial log dilutions (from a starting concentration of ∼ 1.2 × 105 copies / mL) was performed using SPSS 16.0 (SPSS Inc., Chicago, IL).

After acetylation by treatment with 0.1 m triethanolamine 0.25 % acetic anhydride, pH 8.0, the tissue was prehybridized (prehybridization solution: 0.75 m NaCl, 25 mm PIPES, 25 mm EDTA, 5 × Denhardt's reagent, 0.2 % SDS, 250 μg / ml Escherichia coli tRNA, and 500 μg / ml salmon testis DNA, pH 6.8) at room temperature for 5 h. Riboprobes were incubated for 10 min at 85 °C before application onto the sections and used for hybridization at a final concentration of 500 ng / ml.

An LPS - depleted collagenase cocktail (Liberase 3; Roche Applied Science, Indianapolis, Indiana, USA) at a concentration of 0.03 mg / ml and 50 U / ml DNase I (Sigma - Aldrich) was added to the tissue suspension and the samples were incubated at 37 °C on an orbital shaker (215 Hz) for 45 — 60 minutes.

After transformation, a single colony from each was used to inoculate LB medium with Ampicillin (100 µg / ml final concentration).

Spleen cells (1 × 106) were incubated with hamster anti-murine αβTCR (Accurate Chemical, Westbury, NY), hamster anti-murine γδTCR (GL3; kindly provided by Leo LeFrancois, University of Connecticut, Farmington, CT), or PE - conjugated rat anti-Thy 1.2 (PharMingen, San Diego, CA) at a concentration of 1 μg / ml for 30 min on ice, and washed three times in HBSS.

DAPI staining followed the protocol by [11], whereby each section was stained with 100 µl of 4 % PBS buffered paraformaldehyde solution containing 4 ′ 6 - diamidino -2-phenylindole (final concentration 5 µg ml − 1) for 10 minutes, rinsed with filtered 1 × PBS pH 7.4.

Nematode Growth Medium (NGM) agar plates were prepared according to standard protocols and were supplemented with Ampicillin (100 µ g / ml final concentration) and isopropyl β - D -1-thiogalactopyranoside (IPTG)(1.5 mM final concentration).

Neutralises SF2, IIIB, and MN infection at concentrations ranging from 140 ng / ml to 10µg / ml.

Urinary BPA concentrations in 2005/06 (geometric mean 1.79 ng / ml, 95 % CI: 1.64 to 1.96) were lower than in 2003/04 (2.49 ng / ml, CI: 2.20 to 2.83, difference p - value = 0.00002).

Use a concentration of 5 µg / ml.

Bleomycin treated cells were harvested by trypsinization, washed twice and stained with Hoechst 33342 dye (final concentration 5μg / ml) and incubated at 37 °C for 90 minutes.

Human volunteers given an oral dose of 2 g niclosamide exhibited maximal serum concentration of 0.25 - 6 μg / ml and eliminated the drug within 2 days.

A total of 1 × 105 cells were placed in individual wells of a 96 - well plate, and cells were precoated with 100 μl of varying concentrations of plate - bound anti-CD3 (2C11; BD Pharmingen) and 5 μg / ml anti-CD28 Abs in PBS.

In the abyssal realm, seafloor habitats under areas of deep - water formation (e.g., those in the North Atlantic and Southern Oceans) could experience a maximum decline in O2 concentration of 0.03 mL L — 1 by 2100 (i.e., a 0.5 % drop from current levels; Tables 2, 3; Figures 2, 3).

Briefly, the cells were washed in ice - cold PBS and resuspended in binding buffer at a concentration of 1 × 106 cells / mL.

The cells were then incubated with ab7291 at a working concentration of 0.5 μg / ml and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor ® 647, shown in red) at 1/250 overnight at +4 °C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor ® 488 (ab150117) at 2 μg / ml (shown in green).

The section was then incubated with ab7291, 5ug / ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system.

Cells were incubated with control (no PA) or PA - AlexaFluor647 at increasing concentrations (0 — 10 μg / ml).

Protein concentration: Protein was concentrated to ~ 20mg / ml using a 30kDa cut - off concentrator and back diluted to 9 - 11 mg / ml.

Formaldehyde crosslinks were reversed by adding 1 µl of 10 mg / ml RNase and NaCl to a final concentration of 0.3 M and incubation at 65 °C for 4 — 5 hours.

Human tonsillar lymphocytes were treated with increasing concentrations (0.01 — 10 μg / ml) lethal toxin for 3 hrs followed by IL - 23 (50 ng / ml) stimulation for 18 hr.

Protective antigen (PA)- Alexa647 at indicated concentrations: 0 ug / ml (red), 0.01 μg / ml (blue), 0.1 μg / ml (green), 1 μg / ml (orange) and 10 μg / ml (cyan) were used to determine the binding to ILC3 or RAW264.7 mouse macrophages.

The cells were dissociated with 0.25 % trypsin and 1 mM EDTA in PBS (0.25 % trypsin — EDTA) for 10 to 15 min and suspended at a concentration of 100,000 cells / ml in DMEM (Sigma) supplemented with 10 % FBS, 1 mM MEM nonessential amino acids, and penicillin — streptomycin.

The mean Cmax was 114 ng / ml occurring at 1 hour after dosing, and the area under the serum concentration versus time curve was 573 ng × h / ml, with a terminal phase half - life of 1.9 hours observed.

Subconfluent HUVEC cultures were stimulated with PMA (50 ng / mL) for 4 h in the absence (controls) or presence of different concentrations of kahweol.

Where indicated, cells were treated daily with 10 or 200 ng / ml of growth differentiation factor (GDF) 5 (GDF5)(Biopharm GmbH), which are maximal saturating concentrations in primary and SH - SY5Y cell cultures respectively [17,41].

(A) BaF3 cells control or stably transduced with wild - type or with V617F, Y931C or V617F / Y931C mutant were washed 3 times in PBS to remove the residual IL - 3 and seeded in 6 - well plates at a concentration of 100,000 cells / mL in culture medium.

High concentrations are helpful (1mg / ml).

After 24 h, trastuzumab - expressing NSC were selected using increasing concentrations of Zeocin (InvivoGen), beginning at 100 µg / mL.

Breast carcinoma cells (5 × 103 cells / well) were seeded into a 96 - well plate and treated with trastuzumab, isotype - matched control IgG, or F3 - IgG at concentrations ranging from 0.01 to 10 µg / mL.

For sperm count measurements, a sample of sperm was diluted 1/10 in Milli - Q water, and 10 μl were placed on a Bürker chamber to obtain sperm cell concentration (million spermatozoa / ml).

Puromycin and blasticidin were used at the final concentrations of 1.5 μg / ml and 4 μg / ml respectively.

Moreover, the same Kitavans had lower fasting plasma insulin concentrations (2 - 6 IU / mL) compared to healthy Swedes (4 - 11 IU / mL).

The high concentrations of electrolytes in milk (133 mg of sodium and 431 mg of potassium per 250 mL) can increase fluid retention and subsequently improve hydration.

In recent years, The National Academy of Clinical Biochemists indicated that 95 % of individuals without thyroid disease have TSH concentrations below 2.5 μIU / mL, and a new normal reference range was defined by the American College of Clinical Endocrinologists to be between 0.3 - 3.0 μIU / mL.