Sentences with phrase «ml of adenosine»

The assay mixtures were prepared in 25 mM potassium phosphate buffer pH 7.5, 1 mM EDTA, 2 mM MgCl2, 0.01 % Triton X-100 with 5 µM SAHH, 0.3 U / ml of adenosine deaminase from Sigma, 25 µM SAM, and 15 µM Thioglo - 1.