Sentences with phrase «ml of penicillin»

Ticks were placed in snap capped tubes containing a 5 mm stainless steel BB and 1.0 mL mosquito diluent (PBS supplemented with 20 % heat - inactivated FBS, 100 units / ml of penicillin, 100 μg / ml of streptomycin, 10 μg / ml Gentamycin, 1 μg / ml Fungizone).

For virus isolation attempts, serum samples were diluted 1 ∶ 5 in Eagle's minimum essential medium (EMEM) supplemented with 2 % fetal bovine serum, 200 µg of streptomycin, and 200U / ml of penicillin.

Not exact matches

2.5 million CD4 + T cells were seeded at a density of 0.8 × 106 cells / ml in RPMI containing 10 % fetal calf serum, 1 % penicillin / streptomycin, and 100 U / ml interleukin - 2 (IL - 2).

For induction and culture of mouse iPS cells, HFF cells were passaged using trypsin and cultured in medium containing DMEM, 10 % FBS (Hyclone), 1 % NEAA (Invitrogen), 2 mM L - glutamine (Invitrogen), 100 U / ml penicillin and 100 µg / ml streptomycin.

The cells were dissociated with 0.25 % trypsin and 1 mM EDTA in PBS (0.25 % trypsin — EDTA) for 10 to 15 min and suspended at a concentration of 100,000 cells / ml in DMEM (Sigma) supplemented with 10 % FBS, 1 mM MEM nonessential amino acids, and penicillin — streptomycin.

Cells were cultured at 37 °C in an atmosphere of 5 % CO2 in Rosewell Park Memorial Institute (RPMI) 1640 medium (Mediatech, Herndon, VA, USA) containing 10 % fetal bovine serum (FBS)(Gemini Bio-Products, Woodland, CA, USA), 100 U / ml penicillin / streptomycin and 2 mM glutamine (Invitrogen, Carlsbad, CA, USA).

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

HUF cells were propagated in MEF media consisting of DMEM (Invitrogen) supplemented with 10 % FBS (Invitrogen) and 100 IU / ml Penicillin - Streptomycin (Invitrogen).

The MCF10A mammary epithelial cells were cultured in DMEM - F12 medium supplemented with 5 % horse serum, 20 ng / ml EGF, 10 μg / ml insulin, 0.5 μg / ml hydrocortisone, 100 ng / ml of cholera toxin, and penicillin / streptomycin.

I went to a feed store and bought penicillin and injected him (also according to weight) 3 ml of it daily although I know there is no cure this is to prevent any other infection to happen to him.