Sentences with phrase «ml pentobarbital»
Briefly, birds were anesthetized with barbiturate anesthetic, equithesin (intrapectorally: 0.85 g chloral hydrate / 4.2 ml pentobarbital / 0.42 g MgSO4 / 6.92 ml propylene glycol / 1.78 ml 100 % ethanol to a total volume of 20 ml with water, then filtered) and secured on a rotary table.
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Five days after injection, the mice were reanesthetized with pentobarbital (FatalPlus 0.02 ml, i.p; Vortech) and then perfused transcardially with 4 % paraformaldehyde in phosphate buffer (in mM / L: 29 NaH2PO4, 75 Na2HPO4, pH 7.2 — 7.4).