After 6 — 7 days of suspension culture, the neurospheres were replated onto matrigel - coated 60
mm tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.
COS - 7 cells, plated at 70 % confluence in 100 -
mm tissue culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).