Sentences with phrase «model cell lines»

Not exact matches

Also, the NCM460 cell line that Dr. Ebert and the carrageenan lobby criticize is derived from normal colonic mucosa and is useful to model effects on colonic cells, in the same way that other intestinal cell lines, such as Caco2 and HT29, are useful.

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

Capsaicin additionally produced a significant deceleration of the development of prostate tumors created simply by those human cell lines grown in mouse models.

As well as allowing the use of stem cells grown from established cell lines, the technology could enable the creation of improved human tissue models for drug testing and potentially even purpose - built replacement organs.

«This model was trained on genetic data from human tumors in The Cancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers with overactive Ras signaling in an encyclopedia of cancer cell lines,» Greene said.

Today's version of the NPS1 model includes a digital simulation of every building in the area affected by the bomb, as well as every road, power line, hospital, and even cell tower.

The third type — a human osteosarcoma cell line — is a generic model cell.

The study involved laboratory cell lines of human leukemia and mouse models of the disease.

The research, conducted in cell lines and mouse models, explored enhancing the cancer - killing effects of PARP inhibitors not only in regard to AML but also triple - negative breast cancer.

The scientists then tested three new mTOR inhibitors currently under development (pp242, AZD8055 and INK128) in combination with the chemotherapies AraC, Etoposide and Cisplatin to see how they affected laboratory lines of leukemia cells and mouse models of the disease.

The researchers observed the effect of the synthetically produced molecule, JK - 31, on the growth and proliferation of a model human breast cancer cell line and found that it effectively blocked the protein cyclin - dependent kinase 1 (CDK1), which plays a key part in the process of the division of cancer cells, and therefore inhibited the proliferation of the cells.

This is a breast cancer stem cell line 1 (BCSC1) from the newly established cell model.

«Our study results suggest a new drug cocktail that is effective in both human lung cancer cell lines and fly models,» says Cagan.

In human donor cells and mouse experimental models, the researchers demonstrated that TSLP specifically targeted neutrophils — white blood cells that constitute the first line of defense against bacterial infections.

A study combining tumor cells from patients with breast cancer with a laboratory model of blood vessel lining provides the most compelling evidence so far that a specific trio of cells is required for the spread of breast cancer.

In earlier studies involving animal models and human cancer cell lines, researchers found that breast cancer spreads when three specific cells are in direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to spread.

EZH2 inhibition delayed tumor onset in KDM6A - null cells and caused regression of KDM6A - null bladder tumors in both patient - derived and cell line xenograft models.

To conduct their study, Kaur, Baiocchi and their colleagues used tumor tissue from patients, cell lines and an animal model.

Fourteen participating teams each engineered two chemotaxis models, the amoeba Dictyostelium discoideum (Dicty), and the human cell line HL60, to run a millimeter - long maze of interconnected, orthogonal channels designed to mimic the cell's natural environment.

To validate their computer modeling predictions, researchers performed experiments in human cancer cell lines, mouse liver samples and primary human hepatocytes.

«We compared the ability of RSV and parainfluenza virus (PIV3)-- another common virus in children that causes much less severe airway disease — to infect and cause inflammatory responses in a cell culture model of human epithelial cells, which compose the lining of the lung airway.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

In a new study published in Scientific Reports, Tokyo Medical and Dental University (TMDU)- led researchers investigated photodynamic detection of cancer stem cells in a glioma cell line, a model of a highly aggressive type of brain cancer.

Apart from a few studies in mouse models and in cell lines, there is no laboratory evidence that synthetic phosphoethanolamine works as a cancer drug.

Cheng, an assistant professor of medicine in hematology / oncology at Feinberg, provided the cell lines and NanoFlare targets the researchers used to model blood samples taken from breast cancer patients.

One of the most popular models is the immunodeficient nonobese diabetic severe combined immunodeficiency (NOD scid) gamma (NSG) transgenic mouse line, which can be endowed with a humanized immune system using CD34 + hematopoietic stem cells.

Provide example data from experiments using PD - L1 positive tumors and a cancer cell - line engraftment into a humanized mouse model that was used to test anti-PD1 immune therapy

Human tumor tissue or cell lines can be coengrafted into these mouse models, providing a powerful tool for studying the interactions between human immune cells and human cancers.

The researchers hope their new cell lines will be a useful resource for studying the cellular and molecular intricacies of Huntington's further, and suggest they may provide a model for examining other diseases of the brain that are specific to humans.

In experiments with cancer cell lines, the PIM1 inhibitors killed cells in a MYC - dependent manner, and in two different mouse models — one in which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regression.

In a four - year study conducted on the mouse model in advanced breast cancer metastasis in the eye's anterior chamber, Petty and colleagues found that the new nanoparticle not only killed tumor cells in the eye, but also extended the survival of experimental mice bearing 4T1 tumors, a cell line that is extremely difficult to kill.

They show that this new formulation reduces the minimal curative dose in a disease model, based on infections in mice, by 100-fold and, most importantly, circumvents drug resistance in a cell line that is resistant as a result of mutations in the transporter that mediates drug uptake.

Some ethicists suggest that tissue donors deserve a share of the tremendous commercial potential of iPS cell lines as disease models, drug - testing platforms or treatments.

Most of those findings came from studies in cell line models of diseases, however.

The study used whole - exome sequencing to characterize genetic alterations that occur at the single nucleotide level for all genes in 25 cell lines commonly used as models of bladder cancer.

In a paper published online May 17, 2016 in the journal Nature Communications, de Jong and co-first author Koji Taniguchi, MD, PhD, and colleagues, report that treating both ERK1 / 2 and the compensatory pathway, ERK5, concurrently with a combination of drug inhibitors halted colorectal cancer growth more effectively in both mouse models and human colorectal cancer cell lines.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

As a result, cell phone makers do not offer many models that have adequate processing power to run video smoothly, and digital cameras in U.S. cell phones are on the opposite side from the display screen (which would make it impossible to see the person on the other end of the line while exchanging sign language).

By restoring protein encoded by PARD3 levels, both, cell lines and animal models of mice, we observed that regulating de novo polarization of cells, significantly reduced the risk of metastasis.

«By using an animal model to expand tumor cells recently removed from patients, we hoped to re-create more closely what actually happens in patients with pancreatic cancer rather than by using existing artificial cell lines,» said Wei Zhang, Ph.D., an endowed Hanes and Willis Family Professor in cancer at Wake Forest School of Medicine, a part of Wake Forest Baptist, and principal investigator of the study.

In this study, NIH scientists used mouse models to show that anthrax toxin proteins work by specifically targeting the cells that line the inner walls of the blood vessels feeding the tumor.

The results have also been confirmed in cell lines and mouse models.

Experimental models include yeast, mammalian and human cell lines, mutant mouse models and tumour samples.

Indeed, human pluripotent stem cell lines and derivatives, which express a disease - related mutated gene, represent a relevant and pertinent disease cell model to analyse pathological mechanisms through large - scale drug screenings.

However, homozygous XPDXP (XPDR683W) and hemizygous XPDXPCS (XPDG602D) human cells are known to be highly sensitive to UV [19,25], as are cells from a homozygous viable XpdXPCS / XPCS (XPDG602D / G602D) mouse model (Figure 4A, dotted line)[23].

The Polonis lab has also employed both cell line - based model systems, as well as primary cell types from uninfected humans, to investigate virus - antibody - host cell interactions and cross-subtype reactivities amongst the major subtypes of the HIV pandemic.

NeuroStemcell is focused on the identification and systematic comparison of progenitor cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal differentiation, generated either directly from human embryonic stem (ES) cells, from Neural Stem (NS) cells derived from ES cells or fetal brain, from induced Pluripotent Stem (iPS) cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate cells in appropriate animals models.

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

The Joslin team found that intestinal tumors grow just as quickly in a mouse cancer model whose «epithelial» cells lining the inside of the intestine have been genetically modified so that insulin can't activate the cells.

Human primary hepatocytes and immortalized cell lines (e.g., HepaRG) are used to study these disorders, but due to these cells» high variability, low availability, short assay window, or lack of physiological relevance, researchers studying metabolic diseases are searching for more robust and reliable models.