His lab has extensive experience evaluating and modulating T cell responses to tumors and viruses, including introducing genes into T cells to impart specificity and modulate function, designing strategies to overcome tolerance and enhance in vivo activity, and developing mouse models that more accurately
model human immune responses to candidate vaccines.
Not exact matches
The advance, reported in Proceedings of the National Academy of Sciences (PNAS) journal, for the first time allows scientists to analyze how normal gut microbes and pathogenic bacteria contribute to
immune responses, and to investigate IBD mechanisms in a controlled
model that recapitulates
human intestinal physiology.
With our
human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens,
immune cells, and vascular and lymphatic endothelium, as well as
model specific diseases to understand complex pathophysiological
responses of the intestinal tract.»
«Much further work in both
models and
humans is required to fully untangle this complex web of
immune responses.
It has been suspected in
humans, and shown in animal
models, that the host's
immune responses can make disease worse.
Discuss how mouse
models can be used to study
human immune responses against leukemia (using primary or genetically modified leukemia cells) and melanoma
IDMIT will contribute 1) To the development and validation of assays based on flow cytometry and mass cytometry for the evaluation of
immune responses in
humans and animal
models; these tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal
model platform, in particularly by providing access to NHP
models and to new technologies for in vivo imaging infections and host
responses; 3) To networking activities, in particular by organising a workshop on in vivo imaging.
Such
models are valuable for the analysis of parameters associated with vaccine safety, immunogenicity, delivery and protection as NHPs replicate key features of the
human immune response more faithfully than any other animal
model.
Hypothesis driven approaches to vaccinology can utilise the knowledge gained from mechanistic mouse
models and our molecular understanding of intrinsic defects to
human cells.5 However, caution is required when extrapolating data from murine
models, as there are substantial differences between
immune ageing in mice and
humans.6 Nevertheless,
model systems and ex vivo analyses of molecular alterations in aged
human cells have identified multiple changes in the vaccination
response with age and the aged
immune system in general.
Our technological expertise ranges from the most fundamental approaches to study membrane transport in lymphocytes and dendritic cells (subcellular compartmentalization, intravital microscopy, phagosomal functions), the systematic analysis of gene expression and it regulation (RNAseq, Chip Seq, proteomics) and physiological and pathological
immune responses (mouse
models for cancer immunity, immunomodulation / vaccination,
human clinical studies in cancer).
We chose this
model because 1) it more closely recapitulates features of
human pancreatic cancer than do s.c. - implanted tumors, 2) it can be used in immunocompetent mice to permit assessment of
immune responses, and 3) the cells grow in vivo with predictable kinetics (34).
For understanding the biology of gene - gene, gene - drug and gene - microenvironment interactions, a considerably broader range of in vitro and in vivo
model systems is required — we are generating 1,000 organoid cultures from
human cancers, characterising their genomes, functional dependencies and drug
response, and we are expanding our in vivo
models to study the interface between cancer and the
immune system and microenvironment.
Subsequent studies in animal
models that are thought to mimic the
human experience indicate RSV inactivated vaccine induces an increased CD4 + T lymphocyte
response, primarily of Th2 cells and the occurrence of
immune complex depositions in lung tissues [32], [42], [43].
His most recent work, studying
human skin tissues, confirms that these mouse
models mimic the allergic
immune response seen in
humans.
For the first time
immune cells created from
human induced pluripotent stem cells (HiPSCs) have been used to
model immune response variation between people
A few interesting articles in early life
human microbiome, plus: A comparison between Staphylococcus epidermidis commensal and pathogenic lineages from the skin of healthy individuals living in North American and India; A new tool to reconstruct microbial genome - scale metabolic
models (GSMMs) from their genome sequence; The seasonal changes in Amazon rainforest soil microbiome are associated with changes in the canopy; A specific class of chemicals secreted by birds modulates their feather microbiome; chronic stress alters gut microbiota and triggers a specific
immune response in a mouse
model of colitis; and evidence that the short chain fatty acids profile in the gut reflects the impact of dietary fibre on the microbiome using the PolyFermS continuous intestinal fermentation
model.
The department of Immunology has a long tradition in studies of innate, mucosal, and placental, as well as classical acquired
immune responses, in both
human and experimental mouse
models.
Nippostrongylus brasiliensis, a nematode parasite of rodents, has a parasitic life cycle that is an extremely useful
model for the study of
human hookworm infection, particularly in regards to the induced
immune response.
In summary, the TJU team's findings suggest that B. hermsii - infected humanized NSG mice are an excellent
model for studying the pathogenesis of, and
immune responses to,
human relapsing fever.