Sentences with phrase «modified in embryonic stem cells»
Base oxidation regulates gene activity In cooperation with colleagues at LMU, as well as researchers based in Berlin, Basel and Utrecht, Carell and his group have now shown, for the first time, that a standard base other than cytosine is also modified in embryonic stem cells of mice.
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The laboratory process, described in the journal Scientific Reports, entails genetically modifying a line of human embryonic stem cells to become fluorescent upon their differentiation to retinal ganglion cells, and then using that cell line for development of new differentiation methods and characterization of the resulting cells.
The goal of this protocol (written in French) is to describe the steps and quality controls to prepare new stable murine embryonic stem cells lines: ① either from wild type mouse embryos to introduce a mutation by genetic targeting or homologous recombination to get a genetically modified mouse line ② or from genetically modified mouse embryos with two aims: