Sentences with phrase «molecule targeted sequencing»

«Hyb & SeqTM Technology: A no - amp, no - library, single - molecule targeted sequencing approach developed for clinical applications»

Not exact matches

The vaccine can be targeted to the chosen pathogen by using RNA molecules which share sequence identity with the pest's genes and prevents their expression.

The HeliScope Single Molecule Sequencer is the first commercial single - molecule sequencing instrument, so called because it does not require the production of millions of copies of the target DNA for the aMolecule Sequencer is the first commercial single - molecule sequencing instrument, so called because it does not require the production of millions of copies of the target DNA for the amolecule sequencing instrument, so called because it does not require the production of millions of copies of the target DNA for the analysis.

CRISPR — Cas9 uses an RNA molecule to target DNA, cutting to a known, user - selected sequence in the target genome.

But oligomers are large and somewhat clumsy molecules, and they occasionally bind to cellular components other than the target sequence.

Through the combination of CRISPRs and RNA - guided endonucleases, such as Cas9, («Cas» stands for CRISPR - associated), bacteria are able to utilize small customized crRNA molecules (for CRISPR RNA) to guide the targeting and degradation of matching DNA sequences in invading viruses and plasmids to prevent them from replicating.

In RNAi, double - stranded RNA molecules target a messenger RNA with a corresponding sequence and cause it to be chewed up, thus preventing the messenger RNA from making its protein.

It involves adding all reagents in a single reaction: CRISPR - Cas12a and its RNA targeting sequence (guide RNA), fluorescent reporter molecule and an isothermal amplification system called recombinase polymerase amplification (RPA), which is similar to polymerase chain reaction (PCR).

If the beetle does evolve to resist an RNA molecule, he says, geneticists could easily launch a new assault: just «slide the sequence over» by a few letters or target several genes at once.

Although this intriguing possibility remains theoretical, the similarities in the amino acid sequences of NGF and insulin are not great enough to result in any similarity of function: the two molecules have completely different target cells and biological activities.

Jennifer Doudna and Samuel Sternberg used a combination of single - molecule imaging and bulk biochemical experiments to show how the RNA - guided Cas9 enzyme is able to locate specific 20 - base - pair target sequences within genomes that are millions to billions of base pairs long.

In the presence of a donor DNA molecule with homology to the target region, the CRISPR system can also be used to introduce specific nucleotide modifications into a sequence of interest.

Cas9 uses short RNA molecules, known as guide RNAs, to target the protein to a specific sequence within cellular DNA.

These phage libraries have tremendous diversity, so you can use them to find very unique antibody protein sequences that target specific molecules within disease pathways,» says Coyle.