The protocol can be used to measure adaptive responses following the stimulation of whole blood samples or purified peripheral blood
mononuclear cells (PBMCs) and includes a freezing step that allows the stimulated samples to be shipped from the
collection site to the analysis facility.
The blood is efficiently separated so that
mononuclear white blood
cells and peripheral blood stem
cells can be transferred to a
collection bag which will be transferred to the lab and frozen for later use in adult stem
cell therapy.