Sentences with phrase «mouse and human fibroblasts»

Oct4, Sox2, c - Myc, and Klf4 were the four original factors that were capable of converting mouse and human fibroblasts into iPs cells [1], [3], [5], [11], [12].

It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell - like state by introducing combinations of four transcription factors.

Not exact matches

As controls, fibroblasts and secretions from normal lab rats, mice, and another rodent called the spiny mouse were powerless to stop the human cancer cells growing.

In vitro experiments used human colon carcinoma cells with and without MMP9 and mouse embryonic fibroblasts, which are connective tissue cells that make the extracellular matrix and collagen and play an important role in tissue repair.

In a study published recently in the journal Carbon, the team grew human and mouse fibroblast cells (cells involved in wound healing) on flat graphene sheets and on wrinkled ones.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

Despite overexpression of mRNA from the XpdTTD allele relative to the wt allele (Figure 1E), TFIIH protein levels were reduced by 50 % in primary mouse XpdTTD / TTD fibroblasts (Figure 4E and 4F), thereby mimicking the situation in human patients with TTD.

Having shown that it's possible to directly reprogram mouse fibroblasts (in a dish and in adult hearts) and also human heart fibroblasts in a dish, Dr. Srivastava's group is testing the recipe in pigs, whose heart size and physiology is closer to our own.

Yamanaka's group used human adult dermal fibroblasts and induced them to become iPSCs, appearing and functioning like hESCs, by having them express the same proteins as he used with mouse cells: Oct - 4, Sox2, Klf4, and c - Myc (Takahashi et al., 2007).

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

While scientists have successfully reprogrammed different types of mouse cells (fibroblasts, liver and intestinal cells), skin fibroblasts were the only human cell type they had ever tried their hands on.

Pluripotent stem cells can be generated from adult mouse - tail tip fibroblasts and adult human fibroblasts by the retrovirus - mediated transfection of four transcription factors, Oct3 / 4, Sox2, c - Myc, and Klf4.