Sentences with phrase «mouse and human ips»

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

Not exact matches

The group has already started tweaking human iPS cells using the same genes that Saitou pinpointed as being important in mouse germ - cell development, but both Saitou and Hayashi know that human signalling networks are different from those in mice.

The current research team, which includes Dr. Minoda and Dr. Hiroki Takeda of Kumamoto University, and several researchers from Keio University, have successfully grafted human iPS - derived cells into the inner ear of embryonic mice, a feat with a high level of technical difficulty.

The mice show many of the symptoms that human patients do, and so they were an especially good candidate to test iPS cells» abilities, says stem cell researcher Rudolf Jaenisch of the Whitehead Institute for Biomedical Research and the Massachusetts Institute of Technology, both in Cambridge, who collaborated with Townes on the project.

To date, researchers have used human iPS cells to make cardiac cells that repaired heart damage in a pig and insulin - producing pancreas cells that reversed high blood sugar in mice.

Yamanaka's group used human adult skin cells and induced them to become iPS cells by having them produce the same protein factors that the mouse iPS cells had.

Now you make one hundred mice with these human iPS derivatives and ask: do all the mice get diabetes?

Oct4, Sox2, c - Myc, and Klf4 were the four original factors that were capable of converting mouse and human fibroblasts into iPs cells [1], [3], [5], [11], [12].

To our knowledge, this is the first report of generation and maintenance of mouse iPS cells on human feeder cells without exogenous LIF.

Our lab uses both cardiomyoctes derived from human stem cells (iPS cell - derived cardiomyocytes) and mouse models harboring the human mutation to study which exact changes occur during the onset and development of the disease.

Darabi R, Arpke RW, Irion S, et al., Human ES - and iPS - derived myogenic progenitors restore DYSTROPHIN and improve contractility upon transplantation in dystrophic mice.

The immunopurification (IP) of Argonaute (Ago), a central component of the RISC in the human and mouse, followed by microarray analyses (Ago IP / microarray method) makes it possible to isolate any Ago - associated miRNAs and mRNAs without relying on the mechanism of regulation (i.e. mRNA decay or translational suppression), or sequence conservation, enabling a comprehensive identification of the miRNA - target genes in an unbiased manner.