Sentences with phrase «mouse blastocysts»

Gardner DK, Lane MW, Lane M. Development of the inner cell mass in mouse blastocysts is stimulated by reducing the embryo: incubation volume ratio.

Now you know that if you take a mouse embryonic stem cell that is, say, labeled so all of the developed cells will be blue, and you inject it into a mouse blastocyst, you'll get a chimera.

By manipulating a specific gene in a mouse blastocyst — the structure that develops from a fertilized egg but is not yet an actual embryo — scientists with the University of Florida's McKnight Brain Institute and the Harvard Stem Cell Institute caused cells destined to build an embryo to instead change direction and build the cell mass that leads to the placenta.

Not exact matches

But researchers will have to figure out how to eliminate the viral DNA used to introduce the genes, which in Yamanaka's experiments led to cancers in 20 percent of mice grown from blastocysts.

The scientists also injected dye - tagged cells into blastocysts, which they inserted into female mice.

We offer quality custom generation of a wide range of mouse models developed by microinjection of Sv129 / Pas or C57BL / 6N ES cells into blastocysts or by DNA pronuclear injection.

OMC also aims at potentiating the existing Conditional Mutagenesis Facility by implementing novel approaches to attain tissue -, stage - and site - specific transgene expression in mouse mutant strains, mainly through lentiviral vector - mediated transduction of blastocysts.

Then the ES cells have to be injected into a blastocyst, followed by mouse breeding to «go germline.»

Live mice are supplied from a production colony, from a colony recovered from cryopreservation, or via micro-injection of a cell line into host blastocysts.

de Dios Hourcade J, Lopez - Vidriero I, Benguria A, De Fonseca FR, Gutierrez - Adan A. Analysis of gene transcription alterations at the blastocyst stage related to the long - term consequences of in vitro culture in mice

Interestingly, it has been reported that mice with a mutation in Kap1 were obese and had behavioral problems, and the lower expression of Kap1 that we have found in the blastocysts produced by IVC could also be related to the behavior problem reported in mice generated by IVC [43].

The GEM Facility will thaw one straw of frozen embryos and culture overnight to the blastocyst stage to insure that embryo freezing is feasible for that particular mouse line.

Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst.

Histone modifications at the blastocyst Axin1 (Fu) locus mark the heritability of in vitro culture - induced epigenetic alterations in mice

Injection of mouse embryonic stem cells into blastocysts / Service Request form plus documentation.

By contrast, Pou5f1 - null mouse embryos maintained the expression of orthologous genes, and blastocyst development was established, but maintenance was compromised.

Cells with normal karyotype were injected into blastocysts isolated from C57BL / 6 mice.