Sentences with phrase «mouse blood stem»

Scientists from the German Cancer Research Center (DKFZ) have developed a way to equip mouse blood stem cells with a fluorescent marker that can be switched on from the outside.

Not exact matches

The researchers confirmed this hypothesis by showing that if they blocked YAP1 they could inhibit stem cells from undergoing self - renewal, forming blood vessel - like structures, and reduce lung cancer cell growth in mice.

In experiments on normal and MLL cells from mice and humans, the researchers demonstrated that beta - catenin is activated in cancer stem cells that prompt leukaemic blood cells to multiply.

In a study recently published in the journal Nature Biotechnology, HSCI researchers at Harvard University and Massachusetts General Hospital (MGH), in collaboration with Boston Children's Hospital and Dana Farber Cancer Institute, have developed a non-toxic transplantation procedure using antibodies to specifically target blood stem cells in mice, an approach they hope will make blood stem cell transplants for these patients far less toxic.

«When we transplanted our labeled blood stem cells from the bone marrow into other mice, only a few stem cells were active in the recipients, and many stem cells were lost,» Rodewald explains.

Dr. Katrin Busch from Rodewald's team developed genetically modified mice by introducing a protein into their blood stem cells that sends out a yellow fluorescent signal.

Lee conducted a real - time attempt to reproduce a controversial experiment published in Nature in which researchers claimed to have turned adult mouse blood cells into pluripotent stem cells.

During embryonic development of mice, however, the situation is different: To build up the system, all mature blood and immune cells develop much more rapidly and almost completely from stem cells.

In a related paper published online today in Nature Biotechnology, Konrad Hochedlinger of the Harvard Stem Cell Institute in Cambridge and his colleagues compared the gene expression patterns in mouse iPS cells derived from white blood cells, muscle precursor cells, immune system cells called B cells, and fibroblasts taken from tail tips.

The effect can work both ways — young mouse stem cells lose potency in old blood.

Researchers have harnessed the CRISPR - Cas9 technology to correct mutations in the blood stem cells of patients with a rare immunodeficiency disorder; the engineered cells successfully engrafted in mice for up to five months.

Transplants grown from stem cells in the lab can help replenish the blood and have been used to cure anaemia in mice.

2 - D cell - culture and mouse experiments also provided key evidence of the virus's modus operandi; although the rodent brain doesn't harbor the full contingent of human neural stem cells, it has blood vessels and immune - system components that organoids lack.

Both groups isolated carefully characterized blood stem cells from genetically marked mice.

Some of the first evidence for cancer stem cells came from studies of leukemia in the 1990s, which showed that only a small subset of the cancerous blood cells could propagate the disease in mice.

A team from Cold Spring Harbor Laboratory in Long Island, N.Y., reports that it staved off full - blown metastasis in mice by preventing mini-tumors in the lungs from recruiting stem cells called endothelial progenitors, which assemble into blood vessels to nourish the malignancy.

Earlier mouse studies by Li and his collaborators had indicated that the expression of several imprinted genes changes as hematopoietic stem cells embark on their journey from quiescent reserve cells to multi-lineage progenitor cells, which form the many highly specialized cell types that circulate within the blood stream.

A study published January 4th in Cell Stem Cell demonstrates that a gene therapy approach can lead to the long - term survival of functional beta cells as well as normal blood glucose levels for an extended period of time in mice with diabetes.

All it took to make pluripotent stem cells, they reported, was briefly bathing blood cells from newborn mice in a mildly acidic solution and then tweaking the culture conditions.

This rejuvenated the stem cells in the bone marrow of the older mice that replenish their blood, and led to a wave of studies comparing the blood of old and young mice to try and identify the youth - giving substance.

To find out if this was true, workers in stem - cell biologist Irving Weissman's lab at Stanford University Medical School took one blood stem cell from an adult mouse and tagged it with a marker that glowed green under fluorescent light.

A sleep deficit of just four hours affects by as much as 50 percent the ability of stem cells of the blood and immune system to migrate to the proper spots in the bone marrow of recipient mice and churn out the cell types necessary to reconstitute a damaged immune system, the researchers found.

In a matter of weeks, the single stem cell repopulated the mouse's blood and immune system but did not create other types of cells.

A significantly higher amount of stem cells had survived and integrated into the wound tissue in mice that had received celecoxib, and there were fewer inflammatory white blood cells and lower levels of cytokines in their wounds, including one cytokine called interleukin - 17A.

Rolls and her colleagues compared the ability of fluorescently labeled stem cells from sleepy and from rested mice to migrate properly from the recipients» blood into the bone marrow.

Bone marrow from the p16 - deficient mice made 3 times as many blood - forming stem cells as bone marrow from the normal mice did.

The researchers then assessed the prevalence of a kind of immune cell called a myeloid cell, which were derived from the donated stem cells in the blood of the recipient mice, at eight and 16 weeks after transplantation.

In order to learn more about this process, Alexander Skupin and his team treated blood stem cells from mice with growth hormones and then watched closely how these progenitor cells behaved during their differentiation into white or red blood cells.

«We found that TRAF6 overexpression in mouse hematopoietic stem cells results in impaired blood cell formation and bone marrow failure,» said Starczynowski, a member of the Division of Experimental Hematology and Cancer Biology at Cincinnati Children's Hospital Medical Center.

In 2005, Rando and his colleagues published a study in Nature showing that stem cells in several tissues of older mice, including muscle, seemed to act younger after continued exposure to younger mice's blood.

With use of advanced mouse models, she and her team found that blood stem cells without adequate SIRT1 resembled aged and defective stem cells, which are thought to be linked to development of malignancies.

Now, researchers have found that a very small subset of anti-viral immune cells, transplanted along with a donor's blood stem cells, could be enough to fight and even prevent the disease caused by CMV, in research conducted in mice and published Jan 16th in the Journal of Immunology.

The new Mount Sinai study reveals how loss of a protein called Sirtuin1 (SIRT1) affects the ability of blood stem cells to regenerate normally, at least in mouse models of human disease.

Their method relied on briefly bathing blood cells from newborn mice in a mildly acidic solution and then tweaking culture conditions to produce stem cells.

Haruko Obokata of the RIKEN Center for Developmental Biology (CDB) in Kobe, Japan, and colleagues at other Japanese institutions and at Harvard Medical School in Boston reported that simply subjecting blood cells from newborn mice to a moderately acidic environment for 25 minutes and then tweaking culture conditions could generate pluripotent stem cells capable of developing into nearly all of a body's cell types.

Reiser's team used a «humanized» mouse model that utilizes patients» peripheral blood stem cells to communicate signals to mouse bone marrow immature myeloid cells.

Then an experiment in 2005 found that young blood returned the liver and skeletal stem cells of old mice to a more youthful state, and work in 2012 discovered that young blood can reverse heart decline in old mice.

«Stem cell transplants may advance ALS treatment by repair of blood - spinal cord barrier: ALS mice improved with stem cell therapy; first step for science in finding better treatment.&raStem cell transplants may advance ALS treatment by repair of blood - spinal cord barrier: ALS mice improved with stem cell therapy; first step for science in finding better treatment.&rastem cell therapy; first step for science in finding better treatment.»

Finally, the researchers injected these partially differentiated cells into sickle cell mice that had been treated with radiation to kill their own blood stem cells.

Rare stem cells (in red) that give rise to scar - tissue secreting myofibroblast cells, here found near the blood vessels of a mouse kidney (in green).

They then used the so - called induced pluripotent stem cells (IPS cells) to reverse a mouse version of the genetic disorder sickle - cell anemia, which causes normally circular red blood cells to form sickle - shaped, thereby impeding blood flow.

The researchers, led by Benjamin Humphreys, MD, PhD, found that a rare population of stem cells located outside of blood vessels in mice become myofibroblast cells that secrete proteins that cause scar tissue.

When blasted with 10 Gy of irradiation — enough to wipe out all blood stem cells in the bone marrow of normal mice — mutant mice that were unable to fully activate p53 experienced only a modest blood count drop.

Artificial bones produce new blood cells in mice, obviating the need for irradiation to kill off resident hematopoietic stem cells in recipients.

In the second study, a team led by Shahin Rafii at Weill Cornell Medicine in New York City used adult mouse cells as their starting material, and then guided them through several steps — including exposure to some of the same gene - activating proteins — to create mature blood stem cells in a petri dish.

In mice, Adams found one type of bone cell produces important chemicals that boost the number of blood - producing stem cells.

In separate experiments reported in Nature — one with mice, the other transplanting human stem cells into mouse bone marrow — researchers demonstrated techniques with the potential to produce all types of blood cells.

There was scant experimental evidence for this hypothesis until 1994, when John Dick and colleagues demonstrated that leukemia - initiating stem cells (LSCs) present in the blood of leukemia patients may induce acute myelogenous leukemia (AML) when transplanted into severe combined immunodeficient mice (2).

The aorta - gonad - mesonephros (AGM) region in the aortic wall appears to be the most important source of new blood cells, and it has been found to contain numerous hematopoietic stem cells by day 11 of mouse embryonic development.

In an NIH - funded study, scientists were able to direct human stem cells to form networks of tiny blood vessels that can connect to the existing circulation in mice.