Sentences with phrase «mouse embryo culture»
Gardner DK, Rodriegez - Martinez H, Lane M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer.
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The team set up a microscope and video camera over several culture dishes, each containing a single neural progenitor cell taken from a mouse embryo 10 days after fertilization.
Sensory and sympathetic ganglia were dissected out of eight - day - old chick embryos and cultured in a semisolid medium in proximity to fragments of mouse sarcoma - 180 tumors.
For Noggin protein - treated caps, stage 9 animal cap explants were cultured with 500 nM mouse Noggin protein (Sigma - Aldrich, catalogue number N6784) in 0.7 × MMR and 50 µg / ml gentamicin sulphate and grown to sibling embryo stage 15 for transplantation.
They then inserted the spatially oriented human stem cells (human rsPSCs) into specific regions of partially dissected mouse embryos and cultured them in a dish for 36 hours.
We exploit chick and mouse embryo animal models, combined with live imaging, cell and tissue cultures and molecular approaches.
We prefer to culture our cells on mouse embryo fibroblasts (MEFs) but occasionally grow the cells feeder free for particular applications, such as nucleofection, viral transduction or karyotyping.
Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP + pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ).
Scientists at the University of Cambridge have managed to create a structure resembling a mouse embryo in culture, using two types of stem cells — the body's «master cells» — and a 3D scaffold on which they can grow.
For instance, MEF cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only cells at early passages (p2 to p3) are used as feeders for derivation and culture of embryonic stem (ES) and iPS cells.
Differential effects of culture on imprinted H19 expression in the preimplantation mouse embryo
The GEM Facility will thaw one straw of frozen embryos and culture overnight to the blastocyst stage to insure that embryo freezing is feasible for that particular mouse line.
Effect of stem cell activation, culture media of manipulated embryos, and site of embryo transfer in the production of F0 embryonic stem cell mice
Culture of preimplantation mouse embryos affects fetal development and the expression of imprinted genes
It has also been reported that culture and transfer of mouse preimplantation embryos resulted in altered postnatal growth and organ sizing in the offspring that persisted into a second generation, suggesting heritable epigenetic modifications [11].
Thus, although the cells of the inner cell mass of the mouse embryo all express the pluripotency factor Oct - 4, neither the inner cell mass nor cultures of mouse ES cells show uniform expression of the pluripotency factor nanog [6], [7].
One - cell embryos obtained from superovulated female mice mated with (C57BL / 6xCBA) males were cultured in KSOM media (Sigma - Aldrich) supplemented with 10 % FCS (Sigma - Aldrich) for 4 days.