Sentences with phrase «mouse embryonic»

Professor Elly Tanaka and her research group at the DFG Research Center for Regenerative Therapies Dresden — Cluster of Excellence at the TU Dresden (CRTD) demonstrated for the first time the in vitro growth of a piece of spinal cord in three dimensions from mouse embryonic stem cells.

Mouse embryonic stem cells with one allele of Melk inserted with lacZ and neomycin - resistance genes between exon 2 and 3 were obtained from the Knockout Mouse Project (KOMP; ID: CSD33136).

Cells were stained in solution using a mixture of GCTM - 2 (mouse IgM), TG30 (anti-CD9, mouse IgG2a) and Thy1.2 - PE (to gate out any mouse embryonic fibroblasts, BD Bioscience, cat.

Execution of pluripotency requires progression from the naïve status represented by mouse embryonic stem cells (ESCs) to a state capacitated for lineage specification.

Mihail Sarov (Max Planck Institute, Germany): CRISPR / Cas9 - induced disruption of gene expression in mouse embryonic brain

These bovine iPSCs cells kept the normal karyotype and displayed dome shaped clones similar to mouse embryonic stem cells.

His pioneering mouse embryonic stem (ES) cell work in the 1980s, demonstrating germ - line transmission and the great potential of ES cells to generate mice carrying mutations in endogenous genes, established milestones in a field that saw the award of the 2007 Nobel Prize in Physiology or Medicine to Mario Capecchi, Martin Evans, and Oliver Smithies.

96/3: 45 RNF12 is essential for X-inactivation in female mouse embryonic stem cells, is required for female mouse development, and might be a target for future therapies to treat X-linked disorders in females: Evidence from a mouse knockout model.

Here we report that cultures of expanded potential stem cells can be established from individual eight - cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells.

Injection of mouse embryonic stem cells into blastocysts / Service Request form plus documentation.

Wnt / b - catenin / Tcf1 pathway controls mouse embryonic stem cell cycle progression and proliferation by regulating the Ink4 / Arf locus.

hESC lines I6 and H9 were maintained on Matrigel (BD Biosciences, Bedford, MA; http://www.bdbiosciences.com) coated dishes in medium (comprised of Dulbecco's Modified Eagle's Medium / Ham's F12 supplemented with 20 % knockout serum replacement (KSR), 2 mM non-essential amino acids, 4 mM L - glutamine, 0.1 mM β - mercaptoethanol, 50 µg / ml Penn - Strep, and 4 ng / ml of basic fibroblast growth factor) conditioned with mouse embryonic fibroblasts for 24 hours as previously described [9], [10].

We transduced our SSEA3 - positive and SSEA3 - negative populations with the same retroviral vectors, under identical experimental conditions, and seeded the transduced cells onto inactivated mouse embryonic fibroblasts (MEFs).

Here we used genome - saturated mutagenesis to create a biobank of over 100,000 individual haploid mouse embryonic stem (mES) cell lines targeting 16,970 genes with genetically barcoded, conditional and reversible mutations.

Briefly, ES colonies were maintained on mitotically inactivated mouse embryonic fibroblasts (density of 60,000 cells / cm2) in media consisting of DMEM (Invitrogen cat.

Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst.

We also used it to study transition of mouse embryonic stem cells to a specific cell state of interest in Eckersley - Maslin et al Cell Reports 2016.

The Knockout Mouse Project (KOMP & KOMP2) is a trans - NIH initiative that aims to generate a comprehensive and public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

Citation: Schulz H, Kolde R, Adler P, Aksoy I, Anastassiadis K, Bader M, et al. (2009) The FunGenES Database: A Genomics Resource for Mouse Embryonic Stem Cell Differentiation.

Mouse embryonic stem (ES) cells are a popular model system to study biological processes, though uncovering recessive phenotypes requires inactivating both alleles.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

Here we characterise Myst2 / Kat7 / Hbo1 protein interactions in mouse embryonic stem cells by affinity purification coupled to mass spectrometry.

Electroporation of investigator - provided construct into mouse embryonic stem cells / Service Request form plus documentation.

It is based on the definitive books of mouse embryonic development by Theiler (1989) and Kaufman (1992) yet extends these studies by creating a series of three dimensional computer models of mouse embryos at successive stages of development with defined anatomical domains linked by a stage - by - stage ontology of anatomical names.

Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.

W. L. Leung, S. Y. Y. Wong, D. Chan, P. P. L. Tam, K. S. E. Cheah, Loss of procollagen IIA from the anterior mesendoderm disrupts the development of mouse embryonic forebrain.

(A) The expression patterns of germ cell marker genes in monkey testis (5 years old), mouse embryonic fibroblast (MEF), monkey ES cells (ES), and developing EBs (days 3, 7, 14, 21, and 28) were examined using an RT - PCR analysis.

Mouse embryonic skin tissue.

At mouse embryonic day (E) 13.5 (Fig. 4b), PRDM16 protein was expressed throughout the left ventricle but was most prominent in the endocardial and epicardial layers.

RT - PCR analysis of RNA from mouse embryonic and adult gonads using primers corresponding to either exons 3 and 9 (upper panel, 501 - bp band) or exons 3 and 12 (lower panel, 847 - bp and 712 - bp bands) reveals that full - length Bol1 is only expressed in postnatal testes, while Bol2 lacking exon 11 is also detectable at low levels throughout gonadal development in addition to its high adult testis expression.

Vascular endothelial growth factor (VEGF) expressed by neural progenitor cells (NPCs)(cyan) stimulates the growth of blood vessels (red) in the mouse embryonic hindbrain, and provides regulatory factors to promote the self - renewal of neural progenitors.

Shi, Y.; Desponts, C.; Do, J. T.; Hahm, H. S.; Scholer, H. R.; Ding, S. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small - molecule compounds.

Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).

The novel reprogramming approach employed the retroviral transduction of Oct4, Sox2, and Klf4 in mouse embryonic and adult fibroblasts followed by treatment with the GSK3β Inhibitor BIO and the pleiotropic cytokine Oncostatin M (OSM).

InvivoSciences makes engineered heart tissues from mouse embryonic stem cells and stem cells from differentiated adult tissues in humans, such as fat and skin.

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

In their latest study, the group conducted a genome wide screening by developing a novel primary mouse embryonic stem cell line that enhances the efficiency of CRISPR - Cas9 mediated mutations.

What she has shown is you can make mouse embryonic stem cells convert unidirectionally to trophoblasts by activating a single gene.

They made adult mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the adult cells to produce four key protein factors.

TEST CASE RESEARCHER Piroska Szabó, Associate Professor, Center for Epigenetics, Van Andel Research Institute ORGANISM Mouse METHOD RNA - seq In 2014, Szabó and colleagues reported how they'd used mouse embryonic fibroblasts to test whether the relatively new method of RNA sequencing could reveal known and novel imprinted genes.

Nonselective sister chromatid segregation in mouse embryonic neocortical precursor cells.

Dan J, Rousseau P, Hardikar S, Veland N, Wong J, Autexier C, Chen T. (2017) Zscan4 Inhibits Maintenance DNA Methylation to Facilitate Telomere Elongation in Mouse Embryonic Stem Cells.Cell Rep. 20 (8): 1936 - 1949.

Valdivia submitted this image of mouse embryonic fibroblasts forming focal adhesions, points of contact of the cell with the extracellular matrix.

(6) Moreover, subcutaneous adipose of prematurely - aged transgenic mice exhibited high levels of staining for the senescence marker senescence - associated - β - galactosidase (SAβ - gal) and expressed high levels of several established markers of senescence, including p21, p19, interleukin - 6, (insulin - like growth factor binding protein - 2 (Igfbp2), and Pai - 1; primary BubR1H / H; INK - ATTAC mouse embryonic fibroblasts forced artificially into senescence by oncogenic Ras or serial passage exhibited a subpopulation that was both GFP + and stained positively SAβ - gal.

They made adult fibroblastic mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the fibroblasts to express four key embryonic stem cell factors: Oct - 4, Sox2, Klf4, and c - Myc.

With the new SIF - seq technique, mouse embryonic stem cells can be used to identify human embryonic stem cell enhancers even when the human enhancers are not present in the mouse genome.

Endogenous optical signals reveal changes of elastin and collagen organization during differentiation of mouse embryonic stem cells.

The aorta - gonad - mesonephros (AGM) region in the aortic wall appears to be the most important source of new blood cells, and it has been found to contain numerous hematopoietic stem cells by day 11 of mouse embryonic development.

Researchers investigating signaling pathways which control pluripotency highlight newly found interactions and redundancies in mouse embryonic stem cells

Smooth - muscle - specific expression of neurotrophin - 3 in mouse embryonic and neonatal gastrointestinal tract.