Sentences with phrase «mouse embryonic fibroblasts»

Pluripotent cells can be created by introducing transcription factor genes into mouse embryonic fibroblasts on a plasmid that does not integrate into the genome.

For this study, Guttridge, first author David J. Wang, who developed many of the study's concepts, and their colleagues monitored NF - kB activity during tumor development using mouse embryonic fibroblasts and two mouse models.

Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras.

Shi, Y.; Desponts, C.; Do, J. T.; Hahm, H. S.; Scholer, H. R.; Ding, S. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small - molecule compounds.

Briefly, ES colonies were maintained on mitotically inactivated mouse embryonic fibroblasts (density of 60,000 cells / cm2) in media consisting of DMEM (Invitrogen cat.

Valdivia submitted this image of mouse embryonic fibroblasts forming focal adhesions, points of contact of the cell with the extracellular matrix.

Latex bead labelling and comparative immunofluorescence analysis of the p62 subunit of the TFIIH was performed as described previously [16,17] using primary mouse embryonic fibroblasts at passages 2 — 5.

I prefer to culture my hPSCs with mouse embryonic fibroblasts (MEFs) but there are occasions when feeder free growth is required (e.g., nucleofection, viral transduction or karyotyping to name a few).

When transplanted into blastocysts, mouse iPS cells derived from mouse embryonic fibroblasts can give rise to adult chimeras, which are competent for germline transmission.

Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.

TEST CASE RESEARCHER Piroska Szabó, Associate Professor, Center for Epigenetics, Van Andel Research Institute ORGANISM Mouse METHOD RNA - seq In 2014, Szabó and colleagues reported how they'd used mouse embryonic fibroblasts to test whether the relatively new method of RNA sequencing could reveal known and novel imprinted genes.

In vitro experiments used human colon carcinoma cells with and without MMP9 and mouse embryonic fibroblasts, which are connective tissue cells that make the extracellular matrix and collagen and play an important role in tissue repair.

With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.

Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).

Cells were stained in solution using a mixture of GCTM - 2 (mouse IgM), TG30 (anti-CD9, mouse IgG2a) and Thy1.2 - PE (to gate out any mouse embryonic fibroblasts, BD Bioscience, cat.