Sentences with phrase «mouse embryonic stem»

Professor Elly Tanaka and her research group at the DFG Research Center for Regenerative Therapies Dresden — Cluster of Excellence at the TU Dresden (CRTD) demonstrated for the first time the in vitro growth of a piece of spinal cord in three dimensions from mouse embryonic stem cells.

Mouse embryonic stem cells with one allele of Melk inserted with lacZ and neomycin - resistance genes between exon 2 and 3 were obtained from the Knockout Mouse Project (KOMP; ID: CSD33136).

Execution of pluripotency requires progression from the naïve status represented by mouse embryonic stem cells (ESCs) to a state capacitated for lineage specification.

These bovine iPSCs cells kept the normal karyotype and displayed dome shaped clones similar to mouse embryonic stem cells.

His pioneering mouse embryonic stem (ES) cell work in the 1980s, demonstrating germ - line transmission and the great potential of ES cells to generate mice carrying mutations in endogenous genes, established milestones in a field that saw the award of the 2007 Nobel Prize in Physiology or Medicine to Mario Capecchi, Martin Evans, and Oliver Smithies.

96/3: 45 RNF12 is essential for X-inactivation in female mouse embryonic stem cells, is required for female mouse development, and might be a target for future therapies to treat X-linked disorders in females: Evidence from a mouse knockout model.

Here we report that cultures of expanded potential stem cells can be established from individual eight - cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells.

Injection of mouse embryonic stem cells into blastocysts / Service Request form plus documentation.

Wnt / b - catenin / Tcf1 pathway controls mouse embryonic stem cell cycle progression and proliferation by regulating the Ink4 / Arf locus.

Here we used genome - saturated mutagenesis to create a biobank of over 100,000 individual haploid mouse embryonic stem (mES) cell lines targeting 16,970 genes with genetically barcoded, conditional and reversible mutations.

Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst.

We also used it to study transition of mouse embryonic stem cells to a specific cell state of interest in Eckersley - Maslin et al Cell Reports 2016.

The Knockout Mouse Project (KOMP & KOMP2) is a trans - NIH initiative that aims to generate a comprehensive and public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

Citation: Schulz H, Kolde R, Adler P, Aksoy I, Anastassiadis K, Bader M, et al. (2009) The FunGenES Database: A Genomics Resource for Mouse Embryonic Stem Cell Differentiation.

Mouse embryonic stem (ES) cells are a popular model system to study biological processes, though uncovering recessive phenotypes requires inactivating both alleles.

Here we characterise Myst2 / Kat7 / Hbo1 protein interactions in mouse embryonic stem cells by affinity purification coupled to mass spectrometry.

Electroporation of investigator - provided construct into mouse embryonic stem cells / Service Request form plus documentation.

InvivoSciences makes engineered heart tissues from mouse embryonic stem cells and stem cells from differentiated adult tissues in humans, such as fat and skin.

In their latest study, the group conducted a genome wide screening by developing a novel primary mouse embryonic stem cell line that enhances the efficiency of CRISPR - Cas9 mediated mutations.

What she has shown is you can make mouse embryonic stem cells convert unidirectionally to trophoblasts by activating a single gene.

They made adult mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the adult cells to produce four key protein factors.

Dan J, Rousseau P, Hardikar S, Veland N, Wong J, Autexier C, Chen T. (2017) Zscan4 Inhibits Maintenance DNA Methylation to Facilitate Telomere Elongation in Mouse Embryonic Stem Cells.Cell Rep. 20 (8): 1936 - 1949.

They made adult fibroblastic mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the fibroblasts to express four key embryonic stem cell factors: Oct - 4, Sox2, Klf4, and c - Myc.

With the new SIF - seq technique, mouse embryonic stem cells can be used to identify human embryonic stem cell enhancers even when the human enhancers are not present in the mouse genome.

Endogenous optical signals reveal changes of elastin and collagen organization during differentiation of mouse embryonic stem cells.

Researchers investigating signaling pathways which control pluripotency highlight newly found interactions and redundancies in mouse embryonic stem cells

Professor Martinez - Arias and colleagues, supported by the European Research Council and the Wellcome Trust, have reconstructed these early stages of development using mouse embryonic stem cells.

His thesis was entitled «Quantitative description of microRNA target site occupancy in mouse embryonic stem cells and derived cells of neuronal lineage.»

Nearly 30 years ago, two groups independently reported the isolation of mouse embryonic stem cells (mESCs)(Martin, 1981; Evans and Kaufman, 1981).

We are using mouse Embryonic Stem cells to understand how regulatory networks contribute to cellular decision - making processes in mammalian systems.

Studies of stable cell maintenance are being conducted in leukemia stem cells, hematopoietic stem cells, and mouse embryonic stem cells.

The Repository of mouse ES cell lines, strains and vectors generated by the trans - NIH KOMP initiative that aims to generate a public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

In partnership with several international programs, the initial five - year phase of KOMP will reach its goal of creating knockout mouse embryonic stem cell lines for each of the approximately 21,000 protein - coding genes in the mouse genome this year.

Anita Bledau (Anastassiadis, TUD)-- «SET Histone methyltransferases in mouse embryonic stem cells and mammalian development» (2011)

Giovanni Ciotta (Stewart, TUD)-- «Tagging methods as a tool to investigate histone H3 methylation dynamics in mouse embryonic stem cells» (2011)

Using mouse embryonic stem cells, they saw that the process involved three players — Pax3, p53, Mdm2 (Mdm2 being a protein that was already known to play a role in shutting down p53.)

Mouse embryonic stem cells stained for key pluripotency markersGRAZIANO MARTELLO Combining computer science algorithms with biological data has enabled researchers to create a model of the minimal number of factors necessary for mouse embryonic stem cells (ESCs) to self - renew in culture.

Expression of the Wnt inhibitor Dickkopf - 1 is required for the induction of neural markers in mouse embryonic stem cells differentiating in response to retinoic acid.

We discovered that mouse mesenchymal stem cells (MSCs) can either fuse and form heterokaryons (cells with 2 nuclei) with mouse embryonic stem cells (ESCs) or can be invaded by ESCs through the entotic process (when one cell enters into another cell).

To appreciate the difference, consider that the old way involves introducing DNA into mouse embryonic stem cells, and then selecting for the rare cells that take up and incorporate the DNA in the right way.

Researchers identify a network of a dozen transcription factors needed to maintain the pluripotent state of mouse embryonic stem cells.

During the next five years, KOMP2 will transform the knockout mouse embryonic stem (ES) cells into adult mice for 2,500 lines of well - characterized knockout mice strains, and IMPC will create about 2,500 additional knockout mouse strains.

The mouse embryonic stem cell knockout resource provides a basis for the characterization of relationships between genes and phenotypes.

The researchers have illustrated the structure in accompanying videos, which show the intact genome from one particular mouse embryonic stem cell.

These are colonies of mouse embryonic stem cells, where cell nuclei are stained in blue.

Intact genome from one particular mouse embryonic stem cell.

As described in the journal Genes & Development, the researchers identified a new pathway controlling heterochromatin organisation in mouse embryonic stem cells.

«We aren't much wiser than we were a few decades ago» — when mouse embryonic stem cells were first isolated — said Harvard diabetes researcher Douglas Melton.

They accomplished this by using mouse embryonic stem cells to manipulate the mouse genome at the very start of development.

Mouse embryonic stem cells injected into rat brains express the AHD2 protein marker (yellow) characteristic of cells lost in Parkinson's disease.