Sentences with phrase «mouse immunoglobulin»

The antibodies used in this study includes COX - 2 (Cayman Chemicals, Ann Arbor, MI, USA), STAT6 for Western blot (Cell Signaling Technology, Danvers, MA, USA), for immunofluorescence (IF)(BD Pharmingen, San Diego, CA, USA), for ChIP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho - STAT6 (Cell Signaling Technology), p300 for Western blot, (Upstate, Lake Placid, NY, USA), for ChIP (Santa Cruz Biotechnology), normal rabbit IgG (Santa Cruz Biotechnology), mouse immunoglobulin (Ig) G1, κ Ig (BD Pharmingen), glyceraldehyde -3-phosphate dehydrogenase (Advanced ImmunoChemical, Long Beach, CA, USA), Cy3 - conjugated AffiniPure Goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) and PARP (Cell Signaling Technology).

He has established the basic conceptual framework of class switch recombination starting from discovery of DNA deletion (1978) and S regions (1980), followed by elucidation of the whole mouse immunoglobulin heavy - chain locus.

The alterations in the pattern of G reactivity are consistent with the tissue - specific binding of molecules to the mouse immunoglobulin heavy chain enhancer.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins.

Not exact matches

The researchers also found that treating the mice with a molecule called CTL - associated antigen - 4 immunoglobulin (CTLA4Ig) suppressed damage to liver cells infected with hepatitis B virus, suggesting that this might be a potential approach to treatment.

Reporting their study data Sept. 22 in the journal Immunity, scientists at Cincinnati Children's Hospital Medical Center say their findings in mice should also provide insights into new therapeutic strategies and diagnostics for food allergies and anaphylactic shock triggered by the immune antibody IgE (immunoglobulin E).

Mice without gp96 also had higher levels of gut immunoglobulin A, which is associated with chronic inflammation.

The mouse heavy chain immunoglobulin gene contains a tissue - specific enhancer.

Brinster RL, Ritchie KA, Hammer RE, O'Brien RL, Arp B, Storb U. Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice.

Analysis of homozygous mutant chimeric mice: deletion of the immunoglobulin heavy - chain joining region blocks B - cell development and antibody production.

The zebrafish immunoglobulin zeta gene (ighz) and mouse Tcrd, which are proximal to the V gene array, are expressed earlier in development.

Recent studies in mice suggested that antibodies produced by old individuals may be encoded by distinct immunoglobulin (Ig) genes and that the somatic hypermutation process in these individuals is compromised.

The membranes were incubated with mouse sera obtained 3 - 5 weeks after inoculation (1:200 dilution), followed by three wash steps for 15 min each and incubation for 1 - 2 h with peroxidase - conjugated sheep anti-mouse IgG (whole molecule) or goat anti-mouse polyvalent immunoglobulins (1:10,000 dilution)(Sigma - Aldrich).

We also demonstrate that immunoglobulin - secreting NSCs exhibit preferential tropism to tumor cells in vivo, and can deliver antibodies to human breast cancer xenografts in mice.