Sentences with phrase «mouse line appswe»

For this, we co-expressed PLE and the fluorescent protein mCherry in DA neurons using a Cre - dependent recombinant adeno - associated viral vector, rAAV2 / 1 - Synapsin:: FLEX - rev - PLE - 2a - mCherry (2a: ribosomal skip sequence [Donnelly et al., 2001]-RRB- that was targeted unilaterally into the VTA of Slc6a3Cre mice, a mouse line that selectively expresses Cre - recombinase in DA neurons (Zhuang et al., 2005).

For each mouse line, Deltagen provided the mouse strain, but also detailed, objective data on the impact of the specific gene deletion on the mouse's phenotype, which includes appearance, health, fitness, behavior, ability to reproduce, and radiological and microscopic data.

Their collaboration with Charles River regarding the mouse line transfer to our animal facility in Boston was an additional advantage.

For each mouse line, Lexicon provided not only the mouse strain itself, but also detailed, objective data on the impact of the specific gene deletion on the mouse's phenotype, which includes appearance, health, fitness, behavior, ability to reproduce, and radiological and microscopic data.

The GEM Facility will thaw one straw of frozen embryos and culture overnight to the blastocyst stage to insure that embryo freezing is feasible for that particular mouse line.

We then monitored mammary tumour incidence and progression in this mouse line on Slc6a14 + / + and Slc6a14 − / − genetic backgrounds.

Test each mutant mouse line (4,000 mouse lines in the first 5 years, and ultimately up to 20,000) through a broad based primary phenotyping pipeline in all the major adult organ systems and most areas of major human diseases.Through this activity and employing data annotation tools, systematically aim to discover and ascribe biological function to each gene, driving new ideas and underpinning future research into biological systems.

In case of the Sanger Institute, lines of interest can be nominated from the Sanger ES cell collection from which then a KO mouse line will be produced and phenotyped.

If a mouse mutant line was provided by selected applicants for a phenotyping project, sharing of the mouse line is also required and facilitated by the INFRAFRONTIER / EMMA repository.

The technical evaluation of projects may require the provision of additional data such as information on the genetic modification of your mutant mouse line if applicable (e.g. affected gene, MGI ID of the gene, type of mutation, ES - cell line used, genetic background (e.g. number of backcross generations), safety level, description of DNA modification, vector, remaining non-recipient DNA, donor organism), mutant phenotype (s), special housing or care requirements, current sanitary status, and intellectual property rights (who generated the mouse line, owner of the mouse line)

Conclusion: Our results indicate that two different HD mouse models have a similar deficit in response inhibition when tested on the peak interval task, although this deficit was not apparent in the BAC HD mouse line.

The transgenic mouse line APPswe / PS1ΔE9 85 was a generous gift of Dr. J.L. Jankowsky.

The research team on which she works has a unique mouse line wherein a particular gene (Bmal1) is overexpressed specifically in the skeletal muscle.

When researchers express interest in obtaining a certain knockout mouse line, the repositories will send them live mice, frozen embryos, sperm, and / or ES cells, so they can study the mice in their own labs.

Identification of a congenic mouse line with obesity and body length phenotypes.

Overview of mouse production and tech dev - Lauryl Nutter Design QA and allele & mouse line QC pipelines - Lauryl Nutter Mouse line QC - what about off - target mutagenesis?

Wu's group is working on a more sensitive four - color reporter line, but until then, he also uses a two - color reporter mouse line called mTmG.

They utilized the mouse line in which GFP is expressed in cells, but have an active TH promoter.

Depositors are asked to send mice of breeding age to the EMMA node to which the mouse line has been assigned to for embryo or spermatozoa cryopreservation.

The goal of this protocol (written in French) is to describe the steps and quality controls to prepare new stable murine embryonic stem cells lines: ① either from wild type mouse embryos to introduce a mutation by genetic targeting or homologous recombination to get a genetically modified mouse line ② or from genetically modified mouse embryos with two aims:

The establishment of the genetically modified mouse line by breeding with the chimeric mice (Germ Line Transmission (GLT) of the mutation)

To understand how heart muscle cells form trabeculae, Wu's team used a Brainbow mouse line called Rosa26 - Confetti.

Fees for archiving a mouse line for distribution to the research community are based on the specific services requested and the estimated costs of importing, preserving and maintaining the strain at the facility.

In order to study the relationship between telomeres and liver damage, the researchers generated a mouse line deficient in TRF1 protein in the liver, thus leaving the telomeres in hepatic cells unprotected and compromising their function.

To target the virus to GABAergic neurons, the researchers used a genetically engineered mouse line that expresses a marker protein in these specific neurons only.

Half of the mice studied, belonging to a genetic line that suffers a form of Crohn's disease were more affected than the remaining half of mice, which belong to a healthy mouse line.

One of the most popular models is the immunodeficient nonobese diabetic severe combined immunodeficiency (NOD scid) gamma (NSG) transgenic mouse line, which can be endowed with a humanized immune system using CD34 + hematopoietic stem cells.

In total, they carried out over 150 different tests on each mouse line.

To this end, another PhD student in the lab, Ji - Feng Fei, generated a novel transgenic mouse line.

We can use technology like these mouse lines to answer important questions about developmental patterns,» he says.

Over 80 percent of the mouse lines assessed had a characteristic that provided a clue to what the missing gene's role might be.

In order to study gene function, the EUMODIC consortium produced mouse lines which each had a single gene removed.

The consortium had to establish new standardized procedures to generate and assess the mouse lines and a central European database to store all the data.

These mouse lines were then analyzed in mouse clinics, where each line was assessed by a series of tests and investigations, allowing to establish the role of the missing genes.

Various mouse lines have been developed to express proteins that can be used to visualize changes in concentrations of calcium, which increase when neurons become active.

In Tg mice lines, it is not uncommon for the phenotype to gradually soften over time, most likely by a decrease in the number of copies of the transgene.

Consequently, 9 additional CreERT2 mouse lines will be generated by PHENOMIN team and will be made available as soon as possible for the scientific community.

We'll have a post that goes into the mouse genome editing process in a bit more detail in the coming weeks, but, in this post, we will outline a simple method for selecting the guide RNA, validating its efficacy in vitro, and using it in mouse embryos to generate gene modified mouse lines.

Investigating mouse models for biological for research The congress aims to promote the International Mouse Phenotyping Consortium (IMPC) mouse lines, importance of mouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imaging.

EMMA comprises over 6000 mutant mouse lines carrying targeted, transgenic, endonuclease - mediated, induced and other types of mutations.

While these contract agreements are reviewed by the MMRRC's Coordinating Committee, the funding entity proposes the mouse lines for deposition at the MMRRC.

With such a rapid acceleration in the production of mouse lines that could be used to study a wide variety of phenomena, it's possible that we'll learn much more from our favourite mammalian model organisms in the near future.

Consequently, 45 additional new mouse lines will be generated by PHENOMIN team, in order to be available as soon as possible for the scientific community.

The mouse clinics participating in INFRAFRONTIER all contributed to major European phenotyping projects such as the European Mouse Disease Clinic (EUMODIC) project, the first internationally coordinated large scale phenotyping effort, which produced and phenotyped 500 IKMC knockout mouse lines.

The National Infrastructure PHENOMIN is pleased to announce the results of its 6th Call: Generation and Characterization of CreERT2 cell specific promoter driven deleter mouse lines.

A comprehensive and secure set of services for the cryopreservation and recovery of mouse lines including importation, recovery, archiving, and distribution.

Once each knockout mouse is phenotyped, researchers can obtain information on what knockout mouse lines are available and how to order them from the University of California Davis KOMP Repository.

«Our decision to procure these knockout mouse lines and data and make them available to the research community will yield tremendous benefits, both in the short and long terms,» said NIH Director Elias A. Zerhouni, M.D. «This trans - NIH initiative will place important mouse models into the hands of researchers, speeding advances in the understanding of human disease and the development of new therapies.

Now located at The Centre for Phenogenomics, the CMMR provides fee - for - service access to its range of services and the publicly available mouse lines and ES cells in its repository.

The MMRRC is preserving and distributing a portion of these mouse lines obtained under NIH's Deltagen - Lexicon KO Mouse Line Procurement contracts with Deltagen, Inc.and Lexicon Genetics, Inc..