Sentences with phrase «mouse line by»

The establishment of the genetically modified mouse line by breeding with the chimeric mice (Germ Line Transmission (GLT) of the mutation)

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

Development of innovative mouse lines by targeted genome modification (knockin - knockout (KI / KO) approaches).

Not exact matches

Capsaicin additionally produced a significant deceleration of the development of prostate tumors created simply by those human cell lines grown in mouse models.

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Their report, published May 10 in the journal Nature Communications, suggests that among more than a dozen different lines of mice developed around the world to mirror autism caused by mutations to the SHANK3 gene, Duke researchers are the first to create a mouse in which that gene has been completely eliminated.

These effects endured not only throughout the animals» lives but were carried on to the next generation and along the same gender lines: The offspring of dual - parented pups turned in superior performances on tests of cognitive ability and social skills than mice raised by single parents.

In testing on two mouse cell lines, the drug combination reduced the viability of cancer cells by about 50 percent when compared with everolimus alone, according to the findings.

He has found that when a lab mouse misses its daily ration, B. theta consumes the globs of sugary mucus made every day by some cells in the intestinal lining.

These mouse lines were then analyzed in mouse clinics, where each line was assessed by a series of tests and investigations, allowing to establish the role of the missing genes.

Chu achieved this by breeding a genetically modified line of mice that produces large yet well - tolerated quantities of the Cas9 gene scissors.

They have since learned that as many as 40 of those lines may never be fully developed; some may even have been contaminated by mouse cells used to sustain them in the lab.

By functionally linking the signal transduction of melanopsin to the control circuit of the nuclear factor of activated T cells, we have designed a synthetic signaling cascade enabling light - inducible transgene expression in different cell lines grown in culture or bioreactors or implanted into mice.

They show that this new formulation reduces the minimal curative dose in a disease model, based on infections in mice, by 100-fold and, most importantly, circumvents drug resistance in a cell line that is resistant as a result of mutations in the transporter that mediates drug uptake.

One by one, he manipulated the receptor genes, breeding a new line of mice each time.

As in earlier studies, the investigators, led by Ren Hen of Columbia University, first created a line of so - called knockout mice that lacked the gene encoding the receptor protein.

By restoring protein encoded by PARD3 levels, both, cell lines and animal models of mice, we observed that regulating de novo polarization of cells, significantly reduced the risk of metastasiBy restoring protein encoded by PARD3 levels, both, cell lines and animal models of mice, we observed that regulating de novo polarization of cells, significantly reduced the risk of metastasiby PARD3 levels, both, cell lines and animal models of mice, we observed that regulating de novo polarization of cells, significantly reduced the risk of metastasis.

To overcome this, researchers from the University of Texas Southwestern Medical Center and Johns Hopkins University stabilized pri - miRNAs by expressing a dominant negative form of DROSHA, the RNase III enzyme responsible for pri - miRNA cleavage, in a variety of human and mouse cell lines.

IL - 15Rα — KO mice also produced a greater number of wheel revolutions than wild - type C57BL / 6 mice, as depicted by the dashed line in Figure 3B.

In this study, NIH scientists used mouse models to show that anthrax toxin proteins work by specifically targeting the cells that line the inner walls of the blood vessels feeding the tumor.

In Tg mice lines, it is not uncommon for the phenotype to gradually soften over time, most likely by a decrease in the number of copies of the transgene.

Consequently, 9 additional CreERT2 mouse lines will be generated by PHENOMIN team and will be made available as soon as possible for the scientific community.

TTD - associated acanthosis (thicker epidermis, indicated by solid vertical line), pronounced granular layer (indicated by arrows), and sebacious gland hyperplasia (indicated by dotted vertical line) were absent in the epidermis of XpdTTD / † XPCS and wt mice.

Investigating mouse models for biological for research The congress aims to promote the International Mouse Phenotyping Consortium (IMPC) mouse lines, importance of mouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imamouse models for biological for research The congress aims to promote the International Mouse Phenotyping Consortium (IMPC) mouse lines, importance of mouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imaMouse Phenotyping Consortium (IMPC) mouse lines, importance of mouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imamouse lines, importance of mouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imamouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imamouse models phenotyping imaging.

While these contract agreements are reviewed by the MMRRC's Coordinating Committee, the funding entity proposes the mouse lines for deposition at the MMRRC.

The goal of this protocol (written in French) is to describe the steps and quality controls to prepare new stable murine embryonic stem cells lines: ① either from wild type mouse embryos to introduce a mutation by genetic targeting or homologous recombination to get a genetically modified mouse line ② or from genetically modified mouse embryos with two aims:

Consequently, 45 additional new mouse lines will be generated by PHENOMIN team, in order to be available as soon as possible for the scientific community.

KOMP2 and IMPC researchers will begin by creating lines of knockout mice from embryonic stem cells produced by KOMP.

The Repository of mouse ES cell lines, strains and vectors generated by the trans - NIH KOMP initiative that aims to generate a public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

By rewriting a tiny portion of the mouse genetic code, the team developed a genetic line of mice with cells that could, under the right circumstances, produce a powerful protein called caspase when they start secreting p16.

Using studies in both mice and humans, they found that exposure to farm dust increases expression of a protective protein that suppresses the inflammatory immune system by modifying the communication between the lining of the lungs and the immune system.

On the contrary, p21 deficiency delays the onset of radiation - induced T - cell lymphomas, which is in line with the delay in the appearance of spontaneous T - cell lymphomas produced by p21 deficiency in ATM - null mice (32).

To better understand this complex tissues and its functions — and the diseases that affect it — a multicenter team led by researchers at the Broad Institute of MIT and Harvard and Massachusetts General Hospital has released a census of the cells that make up the lining of the small intestine, using gene expression profiles of more than 53,000 individual cells from the mouse gut or gut organoid models.

The process used by NIH to select the mouse lines involved a rigorous scientific review process that evaluated information on the knocked out gene, the reliability of the method used to produce the knockout, and whether the mouse line possesses a «reporter» gene, which enables researchers to analyze the pattern of the knockout gene's expression in various mouse tissues.

To facilitate access by the wider scientific community, mouse lines will be cryopreserved and distributed by the INFRAFRONTIER / EMMA repository.

The MMRRC exists to serve the research community by preserving and distributing biomedically valuable mouse strains and mutagenated cell line collections.

Summary: A day - long symposium for the promotion of IMPC lines and emphasis of the importance of mouse phenotyping & clinical and drug discovery collaboration Attended by: IMGC delegates, clinical researchers & drug discovery researchers Hosted / funded by: RIKEN BioResource Centre

In these mouse lines, gene knockout can be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP - FRT.

If a mouse mutant line was provided by selected applicants for a phenotyping project, sharing of the mouse line is also required and facilitated by the INFRAFRONTIER / EMMA repository.

The congress aims to promote the International Mouse Phenotyping Consortium (IMPC) mouse lines, importance of mouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imaMouse Phenotyping Consortium (IMPC) mouse lines, importance of mouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imamouse lines, importance of mouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imamouse phenotyping & clinical and drug discovery collaboration, to present progresses performed by IMPC with regards CRISPR editing genome, rare diseases, microbiota and ageing pipeline, as well as illustration of examples of scientific projects about «Animal models for human diseases» and recent developments in mouse models phenotyping imamouse models phenotyping imaging.

We found that the plaque burdens among the transgenic lines varied by an order of magnitude: at 15 months of age, the oldest age studied, the median cortical plaque burden in 5XFAD mice was already ∼ 4.5 times that of 21 - month - old Tg2576 mice and ∼ 15 times that of 21 — 24 - month - old rTg9191 mice.

In their latest study, the group conducted a genome wide screening by developing a novel primary mouse embryonic stem cell line that enhances the efficiency of CRISPR - Cas9 mediated mutations.

The core services of INFRAFRONTIER comprise the systemic phenotyping of mouse mutants in the participating mouse clinics, and the archiving and distribution of mouse mutant lines by the European Mouse Mutant Archive (EMMA)(www.infrafrontiermouse mutants in the participating mouse clinics, and the archiving and distribution of mouse mutant lines by the European Mouse Mutant Archive (EMMA)(www.infrafrontiermouse clinics, and the archiving and distribution of mouse mutant lines by the European Mouse Mutant Archive (EMMA)(www.infrafrontiermouse mutant lines by the European Mouse Mutant Archive (EMMA)(www.infrafrontierMouse Mutant Archive (EMMA)(www.infrafrontier.eu).

In a series of lab experiments with cell lines, human xenograft tumors in mice and primary human prostate cancer samples, the researchers demonstrated that miR - 34a inhibits prostate cancer stem cells by suppressing CD44.

The progranulin - insufficient mice used for this study were bred from a line generated by crossing Grn - floxed mice with mice carrying β - actin Cre, which were subsequently back - crossed onto a C57BL / 6J background (Martens et al., 2012; Filiano et al., 2013).

For example, by using histone deacetylase (HDAC) inhibitors, which, by remodeling chromatin structure, are thought to promote gene expression broadly, including for SMN2, numerous groups showed that they could increase SMN protein levels — both truncated and full - length — in mouse models of SMA as well as in patient - derived cell lines.

Thus, upon activation by ActD, AKT becomes an inducer of p53 tumor suppression instead of being a survival factor, as consistently shown in human embryonic kidney cell lines (293 and 293T), human hepatoma cell line (HepG2), and mouse hepatoma cell line (Hepa - 1c1c7).

A third line of evidence showing the potential of kahweol to inhibit overall angiogenesis is provided by the ex vivo model of the mouse aortic ring assay.

Dr. Cole's project, «Mechanistic Derivation of Germ Line Mutation by Genome - Wide Mouse Tetrad Analysis,» will help define the global patterns of de novo germ line mutations and the frequencies at which such mutations occur in mice, with the long term goal of understanding the molecular mechanisms underlying germ line mutagenesis and developing strategies to prevent or treat disorders caused by such mutatiLine Mutation by Genome - Wide Mouse Tetrad Analysis,» will help define the global patterns of de novo germ line mutations and the frequencies at which such mutations occur in mice, with the long term goal of understanding the molecular mechanisms underlying germ line mutagenesis and developing strategies to prevent or treat disorders caused by such mutatiline mutations and the frequencies at which such mutations occur in mice, with the long term goal of understanding the molecular mechanisms underlying germ line mutagenesis and developing strategies to prevent or treat disorders caused by such mutatiline mutagenesis and developing strategies to prevent or treat disorders caused by such mutations.

On the basis of studies on the XMRV - producing human prostate cancer cell lines CWR22Rν1 and CWR - R1 and their progenitor tumour xenograft CRW22, they concluded that XMRV infections were caused by contamination during in vivo passaging in nude mice.