Sentences with phrase «mouse stem cell factor»

For EBI2 transduction of BM - derived cells, BM cells were harvested 4 days after 5 - flurouracil (Sigma) injection and cultured in the presence of recombinant IL - 3, IL - 6, and mouse stem cell factor (SCF)(100 ng / ml; Peprotech; Rocky Hill, NJ).

Not exact matches

«Physical forces are certainly a factor in getting the lung lining to be fully functional,» says Anne Bishop at Imperial College London, who has made alveolar cells from mouse stem cells using growth factors alone.

He and his collaborators found that three transcription factors, when targeted to the pancreas of a living mouse, converted fully formed, non-insulin-producing pancreatic cells into cells that were functionally identical to insulin - producing cells — no stem cells required.

All of the mice produced normal amounts of SOX2 during development, when the transcription factor plays a critical role in the genesis of embryonic and neural stem cells.

«Deletion of a stem cell factor promotes traumatic brain injury recovery in mice.»

A Korean team led by Director of the Center for Genome Engineering Jin - Soo Kim, Institute for Basic Science (IBS) and Professor Dong - Wook Kim at Yonsei University has experimented with hemoplia A-derived induced pluripotent stem cells (iPSCs) and hemoplia mice and found a way to correct this inversion and reverse the clotting factor deficiency that causes hemophilia A.

A group of scientists led by Sebastian Jessberger of the Brain Research Institute showed now that also the stem cells of the adult mouse brain asymmetrically segregate aging factors between the mother and the daughter cells.

Focusing on two proteins known as transcription factors, the researchers showed that genetically removing the two transcription factors, Tcf1 and Lef1, in mice is sufficient to prevent leukemia stem cells from persisting.

Ralph Brinster, part of the team at the University of Pennsylvania in Philadelphia that first cultured sperm stem cells in the lab, has written that culturing stem cells from human sperm is not far off — humans and mice, like other mammals, he says, require similar growth factors.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

The research team selectively overexpressed a transcription factor, Klf9, only in older neurons in mice, which eliminated more than one - fifth of their dendritic spines, increased the number of new neurons that integrated into the hippocampus circuitry by two-fold, and activated neural stem cells.

Researchers identify a network of a dozen transcription factors needed to maintain the pluripotent state of mouse embryonic stem cells.

Mouse embryonic stem cells stained for key pluripotency markersGRAZIANO MARTELLO Combining computer science algorithms with biological data has enabled researchers to create a model of the minimal number of factors necessary for mouse embryonic stem cells (ESCs) to self - renew in culMouse embryonic stem cells stained for key pluripotency markersGRAZIANO MARTELLO Combining computer science algorithms with biological data has enabled researchers to create a model of the minimal number of factors necessary for mouse embryonic stem cells (ESCs) to self - renew in culmouse embryonic stem cells (ESCs) to self - renew in culture.

It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell - like state by introducing combinations of four transcription factors.

«Induction of Pluripotent Stem Cells From Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors,» by Kazutoshi Takahashi and Shinya Yamanaka, Cell, Aug. 25, 2006.

They made adult fibroblastic mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the fibroblasts to express four key embryonic stem cell factors: Oct - 4, Sox2, Klf4, and c - Myc.

By generating mice and NSCs mutated in the Sox2 transcription factor (active in many stem cell types), we are beginning to understand Sox2 functions and molecular targets in neural cells (Favaro et al., Nat.Neurosci.

The finding is based on studies of mice with altered levels of the growth factor PDGF in neural stem cells, which play a key role in the development of the eye.

They made adult mouse cells become essentially mouse embryonic stem cells, in appearance and function, by forcing the adult cells to produce four key protein factors.

Citation: Li C, Yu H, Ma Y, Shi G, Jiang J, Gu J, et al. (2009) Germline - Competent Mouse - Induced Pluripotent Stem Cell Lines Generated on Human Fibroblasts without Exogenous Leukemia Inhibitory Factor.

Research Interests: Molecular control of cell fate from stemness to differentiated skeletal and neuronal cell types; SOX transcription factors; skeletal malformation and degeneration diseases; intellectual disability and autism spectrum disorders; mouse genetic models; human pluripotent stem cell differentiation models in vitro

Studies using a mouse model of type 1 diabetes highlight a potential role for human adipose stem cells in treatment regimens and, further, they reveal a secreted factor which has important therapeutic relevance

Pluripotent stem cells can be generated from adult mouse - tail tip fibroblasts and adult human fibroblasts by the retrovirus - mediated transfection of four transcription factors, Oct3 / 4, Sox2, c - Myc, and Klf4.

By introducing the genes for four factors that turn genes on and off, he induced the skin cells of adult mice to become like embryonic stem cells, which he called induced pluripotent stem (iPS) cells.

They went on to show that Sox10, a factor needed for the formation of skin pigment cells from neural crest stem cells during development, was present at high levels in naevi and melanoma samples obtained from both the mouse model and human patients.

Of note, mouse neural stem cells (NSCs) were reprogrammed using only one (Oct4) or two factors (Oct4 and Klf4), due to the endogenously high expression of pluripotent genes, such as Sox2 and c - Myc, as well as several intermediate reprogramming markers [14], [17], [18].

The pathways that connect expression of stem cell surface glycoconjugates such as the TRA -1-60 / GCTM - 2 antigen, receptors, and growth factors in human and even mouse ES cells with the transcriptional networks that regulate pluripotency remain unclear.

Collectively, these data demonstrate that youthful circulating factors can restore the self - renewal and differentiation potential of aged SVZ stem cells, and this effect can persist for some time after isolation from the mouse brain.