Sentences with phrase «mtef4 gene knockouts»

In our tests, the Gene Knockout Kit gave us greater than 80 percent knock - out rates for seven targets,» shares Shondra Miller, Ph.D., Director, Center for Advanced Genome Engineering at St. Jude Children's Research Hospital, on Synthego's web site.

Gene knockouts in people provide drug safety, effectiveness clues.

«Gene knockout using new CRISPR tool makes mosquitoes highly resistant to malaria parasite: Study highlights the potential of mosquito gene - modification to combat malaria.»

In this study, by using a systemic mtEF4 gene knockout mouse model, researchers found that mtEF4 knockout damages the oxidative phosphorylation function in germ cells of male mice, thus causing male sterility.

Such method is called «Gene knockout.»

An international team of researchers has identified a cause for chronic bad breath (halitosis), with the help of gene knockout mice from the UC Davis Mouse Biology Program.

«Gene knockout: Loss of a gene can be compensated by another gene: Effects of genome interventions depend on the methods used.»

Although protein is highly conserved in evolution, previous mtEF4 gene knockouts in some model organisms did not show significant phenotypic change.

Kohli, Manu, et al. «Facile methods for generating human somatic cell gene knockouts using recombinant adeno ‐ associated viruses.»

The research also demonstrated how these non-random outcomes can be harnessed to produce a desired effect, such as a gene knockout or the reading frame restoration of a disease - causing allele.

«Under this grant, we will utilize Lexicon's proprietary gene knockout technology to study genes that may impact the function of motor neurons to advance treatments for spinal muscular atrophy,» said Brian P. Zambrowicz, Ph.D., executive vice president of research at Lexicon.

According to Dr. Burgess, the team is now working to extend the period that sperm cells can be cultured, so there is more time to select cells that have the foreign gene inserted in specific areas of the genome in what is commonly called a «targeted gene knockout.»

We use transgenes and gene knockouts to study regulatory processes in vivo.

Under the grant, Lexicon will utilize its proprietary gene knockout technology to identify genes that, when knocked out, lead to increased levels of mouse Smn protein.

Meehan et al., (2017) Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

Another potential mechanism is that this gene knockout blocks the interaction between pain receptors and chronic inflammation, a process that is thought to cause harm in old tissues and organs.

TRPV1: Gene knockout of the pain receptor TRPV1 is one of a number of methods of slowing aging and extending life in mice that appears to work through altered insulin signaling.

Programmable nucleases, ZFN, TALEN and RGENs enable gene knockout in cultured cells and organisms by producing site - specific DNA double - strand breaks, whose repair via error - prone non-homologous end joining (NHEJ) or microhomology - mediated end joining (MMEJ) gives rise to frameshift mutations.

In these mouse lines, gene knockout can be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP - FRT.

Cas9 nucleases from Streptococcus pyogenes (SpCas9) have been widely used for both gene knockout and knock - in at the level of single or multiple genes.

Overall, making a human cell line with a gene knockout was straightforward, and is a testament to how far genome engineering has come.

For example, Kuroiwa et al. reported that it took upwards of 21.5 months or longer for producing double - gene knockout cattle [34].

Yeast, bacteria, worms, and flies had long been used for gene knockout studies, and mice with knockout genes had just been developed before Waldman began his experiments.

Here, we report that CRISPR / Cas9 mediated approach can efficiently induce monoallelic and biallelic gene knockout in goat primary fibroblasts.

Awareness about 2 important publications from IMPC consortium 1 - The analysis of IMPC database that uncovers 360 new human disease models has just been published: Meehan et al., (2017) Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.

Transgenic and gene knockout / knockin technologies have become important experimental tools for assigning functions to genes at the level of whole complexity of organism, creating models of genetic disorders, evaluating effects drugs and toxins, thus helping to answer fundamental issues in basic and applied research.

Reconstruction of transgenic and gene knockout and knockin mouse models of human diseases.

Dopamine supersensitivity occurs in schizophrenia and other psychoses, and after hippocampal lesions, antipsychotics, ethanol, amphetamine, phencyclidine, gene knockouts of Dbh (dopamine

Citation: Ni W, Qiao J, Hu S, Zhao X, Regouski M, Yang M, et al. (2014) Efficient Gene Knockout in Goats Using CRISPR / Cas9 System.

The single - gene knockout fibroblasts were successfully used for somatic cell nuclear transfer (SCNT) and resulted in live - born goats harboring biallelic mutations.

Recently, Hai et al. reported generation of single - gene knockout pigs by zygote injection of CRISPR / Cas system [13].

Other groups in England and Japan were also working on creating gene knockouts in human cells, targeting other genes.

Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knockouts / knock - ins, and target validation using the most current technologies.

Since MAOA is an X-linked gene [11], hemizygous males from this family effectively represent functional gene knockouts.

Indeed, several lines of evidence suggest that cytokines are associated with animal and human aggression [102]--[104] and IL - 6 was causally linked to aggression in mice by gene knockout evidence [105].

Not exact matches

The total «knockout» of the gene makes the model more effective for studying SHANK3 - related autism and Phelan - McDermid syndrome in humans, many of whom are missing the gene completely, said senior author Yong - hui Jiang, M.D., Ph.D., an associate professor of pediatrics and neurobiology

The process is painstaking and involves the systematic «knockout» — removal — of relevant genes in stem cell cultures.

The knockout mice, which will be made available to academic researchers at low cost, should help efforts to figure out what newly discovered genes do in the body.

This would seem to prevent researchers from making dog «knockouts» lacking a specific gene or engineering dogs that produce mutant forms of a gene.

The research builds upon the group's work on «knockout humans,» which are naturally occurring mutations that inactivate a certain gene.

Looking forward, the researchers plan to investigate knockout mice lacking the gene that produces AGF to further define its role.

Knockout mice, which completely lacked the gene and so manufactured no mu opiate receptors, were all the more sensitive.

Hundreds of its members, descended from a couple who emigrated from Switzerland six generations ago, carry «knockout» mutations of the gene, called SERPINE1.

To find out what BRCA2 does normally, the team reporting the new results, which includes Allan Bradley of Baylor College of Medicine in Houston and Paul Hasty of Lexicon Genetics Inc. in The Woodlands, Texas, created «knockout» mice in which the gene was inactivated.

Knockouts in this sense doesn't refer to boxing or a stunning appearance, but to natural mutations that inactivate, or «knock out» certain genes.

Because mice lacking both genes would not be born alive, the scientists followed up this lead by making «conditional knockout mice,» in which Esrp1 and Esrp2 activity was normal early in fetal development, but then was switched off in skin epithelial cells.

Studying how the knockout mutations affect those traits and health could point to genes that are potentially safe and effective targets for

In collaboration with Yi Xing from UCLA, the team catalogued and analyzed how gene expression patterns in skin cells differed among the Esrp knockouts and found hundreds of significant changes.

The study suggests that human knockouts could prove valuable evidence for understanding how genes work and for developing drugs.

The researchers bred two types of FOXP2 «knockout» mice: One group was homozygous, meaning the mice carried two copies of the disrupted gene; the second group was heterozygous, with one functional and one defective gene.