Sentences with phrase «multiple gene sequences»

Not exact matches

Each guide RNA might hit a single gene or multiple genes, depending on the particular guide sequence.

Using RNA sequencing, the researchers found multiple genes whose abnormal expression could lead to the high rate of death for cloned embryos, including failure to implant in the uterus and failure to develop a normal placenta.

We examined 13,355 prokaryotic ribosomal RNA gene sequences from multiple colonic mucosal sites and feces of healthy subjects to improve our understanding of gut microbial diversity.

In Class II engagement, Sox9 binds to multiple active enhancer elements through sub-optimal, low - affinity Sox dimeric motifs (DNA sequences), resulting in a high level of transcription of cartilage - related genes.

Researchers analyzed the genetic sequencing data of 718 multiple myeloma patients and found that African - Americans had increased mutations in the genes BCL7A, BRWD3 and AUTS2, while white people had more mutations in the genes TP53 and IRF4.

Using a battery of comparative tests to study the genome sequences of the giraffe and the okapi, the scientists discovered 70 genes that showed multiple signs of adaptations.

His team discovered these special sequences are distributed throughout the human genome, embedded in multiple genes as shown in the study in Cell Cycle.

The transmission of different states of gene expression through multiple cell divisions and across generations has been termed «epigenetic,» since the underlying DNA sequence is identical in both states.

According to Dr. Wong, «This combined strategy provides us with a new tool for reliable molecular analysis of any genes containing multiple copies of highly homologous sequences and should improve the positive finding rate for patients with Lynch syndrome if the PMS2 gene is suspected to be defective.

This is in contrast to the well - characterized VRC01 - like family of CD4 - binding site antibodies identified in multiple HIV - positive donors, which share both a heavy chain V gene precursor and unusual features at a DNA sequence level which could allow sequencing - based identification of other similar antibodies in distinct patients [53].

A multiple sequence alignment of selected genes from all 95 unique, fully - sequenced adenovirus genomes in GenBank and TMAdV is performed and the results displayed as a radial phylogenetic tree.

The ability of Nanopore sequencing to define the complete exonic structure of CACNA1C allows us to identify known and novel gene isoforms, including novel exons and multi-exonic skipping events and their expression across multiple brain regions.

It currently analyses a panel of multiple cancer predisposition genes thanks to new - generation sequencing techniques.

By comparing the effects of multiple sequences, you can determine the extent of off target effects and infer the true biological effect of a gene product.

Uncultured bacterial communities have been studied extensively using Sanger sequencing to determine 16S rRNA gene sequences, and multiple studies have helped optimize methods [24, 25].

For this compilation, I've emphasized publications in which whole - genome or exome sequencing was employed to multiple tumors or cancer cell lines, with the goal of identifying significantly altered genes or pathways in malignant cells.

The idea behind our work in bioinformatics is to build on existing methodologies regarding large - scale data analysis and to develop novel algorithms for processing and merging complex biological data from multiple sources such as gene expression data, sequence information, protein - to - protein interaction data, clinico - pathological data etc..

PULLMAN — Researchers at Washington State University have identified a new class of DNA sequence variation in gene promoter regions that could help control the activity of genes.The novel variations, dubbed «multiple nucleotide length polymorphisms,» or MNLPs, altered transcription of the genes they were associated with as much as 11-fold.

For each of these three genes, the researchers found multiple variants that differed in structural ways, such as whether or not they contained an extra sequence of DNA.

For example, during T - cell development genes encoding the TRB molecules are assembled from multiple polymorphic gene segments that undergo rearrangements and selection, generating Vb — Db — Jb combinations that are unique in both length and sequence.

By sequencing the exomes of multiple individuals, isolating what we'd call «tier 1» variants — Nonsynonymous, nonsense, splice site, or frameshift - indel — and then removing all known common variants from public databases, Dr. Shendure and colleagues can reduce 20,000 gene candidates down to a handful.

Gene expression experiments were conducted with the N. vitripennis AsymCX strain used for genome sequencing [10], which was derived from AsymC by multiple generations of sib - mating.

More than half of mammalian genes generate multiple messenger RNA isoforms that differ in their 3 ′ untranslated regions (3 ′ UTRs) and therefore in regulatory sequences, often associated with cell proliferation and cancer; however, the mechanisms coordinating alternative 3 ′ - UTR processing for specific mRNA populations remain poorly defined.

Using high - throughput sequencing of DNA from river sediment up and downstream from the Indian treatment plant [11] we identified a high prevalence of resistance genes from multiple classes of antibiotics.

Other uses of having a known link between multiple DNA sequencing reads includes studies of structural variations in for instance cancer cells, and when linking functional genes to taxonomic groups in microbial communities.

Importantly, our divergent guide RNAs will also enable global CRISPR gene drive elements to overcome the problem of instability caused by including multiple repetitive guide RNA sequences in the drive cassette [34], which in turn is required in order to overcome drive - resistant alleles [35].

With the completion and publication of multiple fully sequenced Woolly Mammoth genomes, the Church lab is discovering potential cold climate adaptations in different genes.