Forced expression of DDX3 leads to the suppression of colony formation activity of HCC, cervical carcinoma, colon cancer, and
murine fibroblast cells (see Fig. 1A).
To explore the biological function of DDX3 on cell growth control, a colony formation assay was done in several tumor cell lines (HuH - 7, HCT116, and HeLa) and normal
murine fibroblast cells (NIH 3T3) under the selection of G418.
A, DDX3 inhibited the colony formation ability of several tumor and normal
murine fibroblast cells.
Not exact matches
A study by Mo's group in a 2011 issue of Experimental Biology and Medicine (236:604 - 613) showed that normal
fibroblasts are 10-fold more resistant than
murine B16 melanoma
cells to geranylgeraniol - mediated growth suppression.
Individual cultures of 3T3 (a
murine fibroblast line) and endothelioma
cells subjected to identical treatment were used for negative and positive controls, respectively.
HuH - 7 (human HCC), HepG2 (human hepatocellular blastoma), 293T (human embryonic kidney
fibroblast), HeLa (human cervical cancer
cells), NIH 3T3 (
murine fibroblast), and HCT116 (human colon cancer) and its derived
cell line HCT116 p21 − / − (kindly provided by Dr. B. Vogelstein, Howard Hughes Medical Institute, Chevy Chase and the Sydney Kimmel Comprehensive Cancer Center of the Johns Hopkins University School of Medicine, Baltimore, MD; ref.
Since
murine NSCs have been «primed» with several of the factors originally discovered to reprogram
fibroblasts into iPS
cells, they represent an attractive source of starting material for iPS
cell induction studies.