Not exact matches
The CRISPR - Cas9
system offers significant advantages over more traditional, directed
mutagenesis methods, including lower costs, shorter timelines, and the capacity to alter multiple genes simultaneously.
The structural analysis guides further
mutagenesis and biochemical experiments in both reconstituted
systems in vitro and in primary cells, to finely analyze the mechanisms that mediate macromolecular function, or lead to a pathological dysfunction.
Oligonucleotidedirected
mutagenesis with the bacteriophage M13
system (Amersham) was used to introduce five point mutations into the coding regions of tat and rev. Alows stud
Mutations were introduced using the quickchange lighting
system site - directed
mutagenesis kit (Agilent) and were checked by DNA sequencing.
Insertional
mutagenesis using engineered transposons is a potent forward genetic screening technique used to identify cancer genes in mouse model
systems.
Here, we demonstrate the feasibility of classical genetic screening in mammalian
systems by using haploid cells, chemical
mutagenesis and next - generation sequencing, providing a new tool to explore mammalian genetic interactions.