When animals were infected with both S. mutans and either the wild type or defective
mutant yeast strains, the researchers observed clear differences.
An accidental escape hinted at what those scents might be good for: «When returning to the lab after a weekend, I found that a flask with a smelly yeast culture was infested by fruit flies that had escaped from a neighboring genetics lab, whereas another flask that contained
a mutant yeast strain in which the aroma gene was deleted did not contain any flies,» Verstrepen recalls.
Next, like every good yeast researcher should, they turned to the Saccharomyces Genome Database (SGD) to identify 86 genes that when knocked out, caused
the mutant yeast strain to not grow in the absence of an amino acid.
Not exact matches
While biofilm formation was abundant if the wild - type
yeast was used, it was substantially reduced in animals infected with the
mutant strain.
To gain a more detailed understanding of the interaction between the
yeast and the enzyme, the researchers measured the binding strength between various
mutant Candida
strains and GtfB using biophysical methods.
The researchers also engineered a
yeast strain where a
mutant condensin was produced by the cell when it went into figurative labor.
In a
yeast genetic screen, mitochondrial
mutants were highly enriched among
strains that positively respond to caloric restriction.
While studying a
mutant strain of
yeast, Horwich said he found himself in a research dilemma that became a pivotal point in his career.