The cell was created by stitching together the genome of a goat pathogen called Mycoplasma
mycoides from smaller stretches of DNA synthesised in the lab, and inserting the genome into the empty cytoplasm of a related bacterium.
Not exact matches
These cells that result
from genome transplantation are phenotypically identical to the M.
mycoides LC donor strain as judged by several criteria.
Intact genomic DNA
from Mycoplasma
mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol — mediated transformation.
We report the design, synthesis, and assembly of the 1.08 — mega — base pair Mycoplasma
mycoides JCVI - syn 1.0 genome starting
from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M.
mycoides cells that are controlled only by the synthetic chromosome.
(LA JOLLA, CA)-- August 4, 2016 — Researchers
from the J. Craig Venter Institute (JCVI) and Synthetic Genomics, Inc. (SGI) have published research describing a method for engineering Mycoplasma
mycoides 16S ribosomal RNA (rRNA) using a one - step process that combines CRISPR / Cas9 gene editing system with yeast recombination machinery.