For example, clusters containing genes that are upregulated during the course of ES cell differentiation (Table 3) include in order of time of expression: cluster 30 that represents genes which take part in the formation of the three embryonic germ layers during gastrulation, i.e., Goosecoid, Cerberus like 1 homolog, Wnt3, Mesp1, Mixl1, mEomes and Even - skipped 1; cluster 15 containing molecular regulators of early mesoderm development including Bmp2, Bmp5, Msx1, Msx2, Cripto, Tbx20, Hey2, Smad6, Vegfr2 (Kdr), Foxf1 and Hand1; cluster 20, which comprises regulatory and structural genes linked to hemopoiesis such as Gata1, Nfe2, Klf1, Tie1, hemoglobins (Hba - x, Hbb - b1) and Glycophorin A; cluster 12, which is rich in genes involved in cardiac development, e.g., Mef2c, Myl4, cardiac Troponin T2, Tropomodulin 1,
myosin binding protein C, Bves, Angiopoietin 1 and Angiopoietin 2; and, cluster 4, which consists mostly of genes associated with neuronal development and differentiation, for example, Neurog1, Neurog2, Olig2, Nkx6.1, Neurod4, Pou3f2, Pou3f4, Cacna2d3, Cacng4, Kcnq2 and EphA5.
These findings suggest that the therapeutic protein was able to interact normally with its
binding partners among the USH1 molecular complex (the proteins cadherin 23, protocadherin 15,
myosin VIIA and harmonin), as required for the mechanoelectrical transduction apparatus of the hair bundle to function correctly.
Although the anesthetics became
bound to all of the various proteins, the most extensive
binding was observed in three proteins, including actin and
myosin - the muscle force producing proteins.
In our second study we found that heterozygous mutations in genes encoding β -
myosin heavy chain (MYH7), α - cardiac actin (ACTC1), cardiac troponin T (TNNT2), cardiac
myosin -
binding protein C (MYBPC3), and alpha - tropomyosin (TPM1) account for 30 % of cases of isolated LVNC in adult patients (Probst et al., 2011).