This was done using shRNA technology specific for BRCA1 in
human myotubes (skeletal muscle fiber cells).
A: The GS activities measured at 0.1 mmol / l in the basal state and in the stimulated state in
myotubes precultured at the indicated insulin concentration.
Our dose - response curve at chronic high insulin levels allowed us to differentiate between a primary defect (probably genetic) and the induction of secondary insulin resistance in
myotube cultures due to hyperinsulinemia.
Within days, the neural stem cells began to make proteins typical of muscle cells, and even joined with the muscle cells to
form myotubes, tubes of fused cells that make up the bulk of living muscle.
Survival motor neuron protein deficiency impairs
myotube formation by altering myogenic gene expression and focal adhesion dynamics.
In
developing myotubes, amphiphysin 2 and caveolin - 3 segregated in tubular and vesicular portions of the T - tubule system, respectively.
Philipp Weissert (Tanaka, MPG)-- «Bmp proteins in
urodele myotube cell cycle re - entry and in regeneration» (2008)
Nuclear movement
during myotube formation is microtubule and dynein dependent and is regulated by Cdc42, Par6 and Par3.
Werner Straube (Tanaka, MPG)-- «Purification of a serum factor that triggers cell cycle re-entry in differential
newt myotubes» (2006)
Myotubes established from type 2 diabetic subjects express a reduced GS mRNA and protein compared with control subjects (5).
Satellite cells are quiescent cells that have to be activated in vivo before proliferating and differentiating
into myotubes.
Preculturing
myotubes for 4 days at increasing insulin concentrations did not change the basal FV0.1 in diabetic or control cultures (Figs. 3A and B).
In this context, cultures of primary human
myotubes offer excellent material for performing studies under standardized conditions.
Diabetic myotubes expressed a reduced basal glucose uptake compared with control cultures when precultured at equal low - insulin concentrations, and the increase in fractional velocity of GS was reduced after acute insulin stimulation at identical glucose uptake rates in diabetic cultures precultured at low insulin concentrations.
Consistent with these findings, satellite cells from SMA mice differentiate prematurely both in vivo and in vitro and form
fewer myotubes.
Skeletal muscle tissue engineering: methods to form
skeletal myotubes and their applications.
Following the generation of iMPCs by induced Pax7 overexpression in hPSC - derived mesoderm cells, Rao et al. employed two - dimensional (2D) conditions to generate a monolayer culture containing two essential components of skeletal muscle tissue: spontaneously contracting
multinucleated myotubes and Pax7 expressing muscle stem cell - like cells.
Clinical research on Urolithin B in mice found that it
enhanced myotubes growth and differentiation by increasing protein synthesis.
Along those lines, it was also recently shown that ROS function as important signaling molecules for muscle hypertrophy in vitro, where it was found that IGF - 1 induced hypertrophy
of myotubes in culture is suppressed by antioxidants (15).
Overexpression of FOXO3A in
mouse myotubes also significantly up - regulates MAFbx expression (49).
Amino acids and insulin act additively to regulate components of the ubiquitin - proteasome pathway in C2C12 myotubes
However, further investigations are needed to clarify whether previous metabolic influences at the level of quiescent satellite cells in vivo can induce irreversible metabolic changes in cultured
human myotubes.
B: The GS activities measured at 10.0 mmol / l in the basal state and in the stimulated state in
myotubes precultured at the indicated insulin concentration.
Our «biased» profiling is based on an in vitro model of mouse terminally
differentiated myotubes, induced to re-enter the cell cycle by the E1A oncogene (23).
Myotubes were precultured in increasing insulin concentrations for 4 days and subsequently stimulated acutely by insulin.
The main difference between the previously described satellite cell cultures and the cultures we used in the present study are that we exposed our culture to the experimental conditions after differentiation to
myotubes had been terminated, at day 4 (1).
Moreover, the satellite cells were subsequently allowed to differentiate to
myotubes, which changed their protein expression and metabolism.
Through that effort it was observed that
myotubes in culture were bigger after incubation with Urolithin B.