Preculturing
myotubes for 4 days at increasing insulin concentrations did not change the basal FV0.1 in diabetic or control cultures (Figs. 3A and B).
Not exact matches
This was done using shRNA technology specific
for BRCA1 in human
myotubes (skeletal muscle fiber cells).
In vitro experiments in myogenic cells initially suggested that IL - 15 was an anabolic factor
for skeletal muscle, able to stimulate the accumulation of contractile protein in differentiated
myotubes (8 — 10).
Myotubes were precultured in increasing insulin concentrations
for 4 days and subsequently stimulated acutely by insulin.
In this context, cultures of primary human
myotubes offer excellent material
for performing studies under standardized conditions.
Along those lines, it was also recently shown that ROS function as important signaling molecules
for muscle hypertrophy in vitro, where it was found that IGF - 1 induced hypertrophy of
myotubes in culture is suppressed by antioxidants (15).