Staining with this multi-color staining workflow yields similar localization patterns as cells stained for one target (compare
nanobodies staining in figure 4A vs 4B).
Some GFP
nanobodies stain poorly when tagged with a Spaghetti Monster because they recognize this GFP scaffold itself.
Not exact matches
Figure 2: Condensin subunit SMC4 tagged with mEGFP and
nanobody -
stained in a prometaphase HeLa cell visualised by an astigmatic 3D STORM microscope.
See figure 4A below for comparison of this one - step
staining with
nanobodies vs two - step
staining with antibodies.