Sentences with word «nanopore»

Detection of active transposable elements in Arabidopsis thaliana using Oxford Nanopore Sequencing technology
I will also describe our plans to to use Oxford Nanopore sequencing as part of our strategy to assemble the very challenging mega-genomes of two of the largest trees on Earth: the giant sequoia, Sequoiadendron giganteum (11 Gb) and the Coast Redwood, Sequoia sempervirens (33 Gb).
IONiseR provides tools for the quality assessment of Oxford Nanopore MinION data.
The MinION is a portable single - molecule DNA sequencing instrument that was released by Oxford Nanopore Technologies in 2014, producing long sequencing reads by measuring changes in ionic flow when single - stranded DNA molecules translocate through the pores.
I will review recent advances in the use of nanopore sequencing for clinical microbiology and human genetics, including our collaborations on viral and bacterial diagnostic sequencing, real - time surveillance, direct RNA and human whole - genome sequencing, and discuss the opportunities and barriers around moving to sequencing as a routine test in the clinic.
High - quality de novo genome assembly of the Dekkera bruxellensis UMY321 yeast isolate using Nanopore MinION sequencing
Here, we describe the results obtained from our current long read sequencing project of 100 Icelanders with Oxford Nanopore technology.
Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION / Illumina hybrid data assembly approaches
They then modified the industrial polyethylene by treating it with benign chemicals to enable water vapor molecules to evaporate through nanopores in the plastic, said postdoctoral scholar and team member Po - Chun Hsu, allowing the plastic to breathe like a natural fiber.
They are also being explored for nanopore sequencing applications.
«Analyzing - omic Instability in breast cancer with nanopore sequencing of patient - derived organoids»
It is specifically tailored for Oxford Nanopore Reads
This talk demonstrates that by moving proteins through nanopores, it is possible to separate these proteins transiently from other macromolecules in solution.
Solid - state nanopore devices, which are made of thin solid - state membranes, offer advantages over their biological counterparts — they can be more easily shipped and integrated with other electronics — but the basic demonstrations of proof - of - principle sensitivity to different DNA bases have been slower.
Sara Goodwin of Coldspring Harbour Laboratory talks to the Oxford Nanopore Community at London Calling.
We have assembled de novo the Escherichia coli K - 12 MG1655 chromosome in a single 4.6 - Mb contig using only nanopore data.
This preclinical study used a solid - state nanopore sensor as a tool for the analysis of hyaluronic acid (HA).
Reported here is the de novo assembly of a eukaryotic genome using only MinION nanopore DNA sequence data by examining a novel Galdieria sulphuraria genome: strain SAG 107.79.
A novel diagnostic method for malaria using loop - mediated isothermal amplification (LAMP) and MinION ™ nanopore sequencer
A reference bacterial genome dataset generated on the MinION ™ portable single - molecule nanopore sequencer
We report a third - generation sequencing assay on nanopore technology (MinION) for detecting BCR - ABL1 KD mutations and compare the results to a Sanger sequencing (SS)- based test in 24 Philadelphia - positive (Ph +) leukemia cases.
These so - called nanopore sequencers, produced so far by a single company, have suffered from poor accuracy.
Early MinION ™ nanopore single - molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples — Virginie Sauvage — PLOS ONE
This presentation will review methods for which Nanopore sequencing and real - time antimicrobial resistance gene analysis can help to address the threat of CRO as a diagnostic tool in the Medical Microbiology Laboratory.
The NHGRI grant will help fund Drndić's further research into the feasibility of graphene nanopore sequencing as a cost - effective alternative to today's methods.
Rapid draft sequencing and real - time nanopore sequencing in a hospital outbreak of Salmonella
The research uses nanopore long - read sequencing to generate the first complete and accurate linear map of a human Y chromosome centromere.
Species level resolution of 16S rRNA gene amplicons sequenced through the MinION ™ portable nanopore sequencer
Oxford Nanopore holds an exclusive license on the use of the pore, Brown said today, and has developed more than 700 variations of it.
In an 8 March letter to the U.S. International Trade Commission, the company denies all charges of infringement, and claims that Illumina is acting on «unsubstantiated speculation» to block Oxford Nanopore products and maintain its own monopoly over conventional DNA sequencing.
Drndić and her colleagues tested their silicon nitride nanopore on homopolymers, or single strands of DNA with sequences that consist of only one base repeated several times.
Speed, single - base sensitivity and long read lengths make nanopores a promising technology for high - throughput sequencing.
In this contribution, we give an example of how the controlled nanopatterning of TiO2 surfaces by BCP nanopores allows for experiments which give insight into the interactions between biological units and the nanopatterns.
This study attempts to extend the use of nanopore sensors into living cells.
Oxford Nanopore introduces DNA «strand sequencing» on the high - throughput GridION platform and presents MinION, a sequencer the size of a USB memory stick
But when the polymer comes into contact with G - series nerve agents — the family of toxic chemicals that includes sarin gas — it flattens into a dense sheet that seals over the carbon nanopores underneath.
The UW team has been exploring nanopore technology to read DNA sequences quickly.
Oxford Nanopore also responded directly to Illumina's suit this week.
The company said it could not only electrically read out the full sequence of nucleotides in DNA as they streamed through an individual pore, but that by early 2013 it would be selling machines with thousands of nanopores running in parallel, making it possible to sequence a full genome in as little as 15 minutes, for around $ 1000.
Oxford Nanopore responds to new, meritless lawsuit, delivered by PacBio one day after GridION launch.
Borla Performance Industries, a leader in the design and manufacture of stainless steel performance exhaust, has an option to license a novel nanopore membrane technology developed at Oak Ridge National Laboratory (ORNL).
Applying Nanopore Sequencing and Real - Time Antimicrobial Resistance Gene Analysis to Address the Threat of Carbapenem - Resistant Gram - Negative Organisms
Nanopore strand sequencing is uniquely suited to analysis of long DNA fragments and base modifications.
Reviewers had expertise in fields ranging from analytical chemistry and nanopore sensing to phytoplankton biology and ecological modeling.
Gundlach and his team, in the process of investigating nanopore sequencing, tried out a variety of molecular motors to move DNA through the pore.
Activated carbon, which is a type of carbon material containing numerous nanopores, is often used to adsorb gases in the energy sector.
As can happen in the scientific process, they developed this tool — the single - molecule picometer - resolution nanopore tweezers, or SPRNT — while working on a related project.
Using a portable, miniature DNA sequencing device (Oxford Nanopore MiniON), the researchers show for the first time that not only can the tool be used for examining environmental samples in extreme and remote settings, but that it can be combined with other methodology to detect active microbial life in the field.
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