Nanopore Long - Read RNAseq Reveals Widespread Transcriptional Variation Among the Surface Receptors of Individual B cells
Not exact matches
This study aimed to assess the feasibility of using the Oxford
Nanopore Technologies (ONT) MinION
long - read sequencer in reconstructing fully closed plasmid sequences from eight Enterobacteriaceae i...
Here, we describe the results obtained from our current
long read sequencing project of 100 Icelanders with Oxford
Nanopore technology.
Results The assembly of
long reads generated using the Oxford
Nanopore MinION ® instrument is challenging as existing assemblers were not implemented to deal with
long reads exhibiting close to 30 % of errors.
New generation of sequencing technology uses
nanopores to deliver ultra
long read length single molecule sequence data, at competitive accuracy, on scalable electronic GridION platform.
Ars Technica reports that with the
long files, «some analysis software couldn't work with the
nanopore reads at all.»
The
long sequencing reads produced by Oxford
Nanopore's platforms enable the assembly of genomes with superior contiguity compared to those produced by second generation technologies.
Nanopore sequencing is a promising technique for genome sequencing due to its portability, ability to sequence
long reads from single molecules, and to simultaneously assay DNA methylation.
The Oxford
Nanopore MinION is a portable single - molecule DNA sequencer that can sequence
long fragments of genomic DNA.
Speed, single - base sensitivity and
long read lengths make
nanopores a promising technology for high - throughput sequencing.
Multi-locus and
long amplicon sequencing approach to study microbial diversity at species level using the MinION ™ portable
nanopore sequencer
The MinION is a portable device that uses
nanopore technology and can sequence
long DNA molecules.
Motivation: The Oxford
Nanopore MinION sequencer, currently in pre-release testing through the MinION Access Programme (MAP), promises
long reads in real - time from a cheap, compact, USB device.
The MinION is a portable single - molecule DNA sequencing instrument that was released by Oxford
Nanopore Technologies in 2014, producing
long sequencing reads by measuring changes in ionic flow when single - stranded DNA molecules translocate through the pores.
Unlike other methods, which read chunks of DNA perhaps a hundred or so base pairs in length, the MinION can read sequences as
long as 882,000 base pairs in length using the
nanopore technology.
Background: Oxford
Nanopore Technologies Ltd (Oxford, UK) have recently commercialized MinION, a small single - molecule nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter of
Nanopore Technologies Ltd (Oxford, UK) have recently commercialized MinION, a small single - molecule
nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter of
nanopore sequencer, that offers the possibility of sequencing
long DNA fragments from small genomes in a matter of seconds.
Long - read sequencing technologies such as Pacific Biosciences and Oxford Nanopore MinION are capable of producing long sequencing reads with average fragment lengths of over 10,000 base - pairs and maximum lengths reaching 100,000 base - pa
Long - read sequencing technologies such as Pacific Biosciences and Oxford
Nanopore MinION are capable of producing
long sequencing reads with average fragment lengths of over 10,000 base - pairs and maximum lengths reaching 100,000 base - pa
long sequencing reads with average fragment lengths of over 10,000 base - pairs and maximum lengths reaching 100,000 base - pairs.
Oxford
Nanopore Technologies» MinION has expanded the current DNA sequencing toolkit by delivering
long read lengths and extreme portability.
A new hybrid approach for MHC genotyping: high - throughput NGS and
long read MinION
nanopore sequencing, with application to the non-model vertebrate Alpine chamois (Rupicapra rupicapra)
Motivation:
Nanopore sequencing may be the next disruptive technology in genomics, due to its ability to detect single DNA molecules without prior amplification, lack of reliance on expensive optical components, and the ability to sequence very
long fragments.
Detection of subclonal L1 transductions in colorectal cancer by
long - distance inverse - PCR and
Nanopore sequencing
Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very
long Oxford
Nanopore reads.